SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor
Markus Hoffmann,Hannah Kleine-Weber,Simon Schroeder,Nadine Krüger,Tanja Herrler,Sandra Erichsen,Tobias S. Schiergens,Georg Herrler,Nai Huei Wu,Andreas Nitsche,Marcel A. Müller,Christian Drosten,Christian Drosten,Stefan Pöhlmann +13 more
TLDR
It is demonstrated that SARS-CoV-2 uses the SARS -CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming, and it is shown that the sera from convalescent SARS patients cross-neutralized Sars-2-S-driven entry.About:
This article is published in Cell.The article was published on 2020-04-16 and is currently open access. It has received 15362 citations till now. The article focuses on the topics: Proteases.read more
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Patient-derived mutations impact pathogenicity of SARS-CoV-2
Hangping Yao,Xiangyun Lu,Qiong Chen,Kaijin Xu,Yu Chen,Linfang Cheng,Fumin Liu,Zhigang Wu,Haibo Wu,Changzhong Jin,Min Zheng,Nanping Wu,Chao Jiang,Chao Jiang,Lanjuan Li +14 more
TL;DR: Direct evidence is provided that the SARS-CoV-2 has acquired mutations capable of substantially changing its pathogenicity, and these viral isolates show significant variation in cytopathic effects and viral load when infecting Vero-E6 cells.
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Interaction of SARS-CoV-2 and Other Coronavirus With ACE (Angiotensin-Converting Enzyme)-2 as Their Main Receptor: Therapeutic Implications.
TL;DR: The current understanding of the interaction of SARS-CoV-2 with ACE2 is reviewed as it has rapidly unfolded over the last months with clear therapeutic implications.
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Identification of Common Deletions in the Spike Protein of Severe Acute Respiratory Syndrome Coronavirus 2.
Zhe Liu,Huanying Zheng,Huifang Lin,Mingyue Li,Runyu Yuan,Jinju Peng,Qianling Xiong,Jiufeng Sun,Baisheng Li,Jie Wu,Lina Yi,Xiaofang Peng,Huan Zhang,Wei Zhang,Ruben J.G. Hulswit,Nicholas J. Loman,Andrew Rambaut,Changwen Ke,Thomas A. Bowden,Oliver G. Pybus,Oliver G. Pybus,Jing Lu +21 more
TL;DR: There may be distinct selection pressures on SARS-CoV-2 replication or infection in vitro and in vivo; an efficient mechanism for deleting this region from the viral genome may exist, given that the deletion variant is commonly detected after two rounds of cell passage.
Journal ArticleDOI
Expression of SARS-CoV-2 Entry Molecules ACE2 and TMPRSS2 in the Gut of Patients With IBD.
Juan Burgueño,Adrian Reich,Hajar Hazime,Maria A. Quintero,Irina Fernandez,Julia Fritsch,Ana M. Santander,Nivis Brito,Oriana M. Damas,Amar R. Deshpande,David H. Kerman,Lanyu Zhang,Zhen Gao,Yuguang Ban,Lily Wang,Judith Pignac-Kobinger,Maria T. Abreu +16 more
TL;DR: The viral entry molecules ACE2 and TMPRSS2 are expressed in the ileum and colon and had high expression in intestinal epithelial cells in animal models of IBD, providing reassurance for patients with IBD.
Journal ArticleDOI
Aging, Male Sex, Obesity, and Metabolic Inflammation Create the Perfect Storm for COVID-19.
TL;DR: This Perspective explores the evolving epidemiological, clinical, biological, and molecular evidence to propose an unfolding paradigm in which old age, chronic metabolic disease (such as obesity, type 2 diabetes, and metabolic syndrome), and male biological sex produce a deadly symbiosis of dysregulated immunometabolism and chronic systemic inflammation that intensifies virally induced hyper inflammation associated with SARS-CoV-2 infection.
References
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