SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor
Markus Hoffmann,Hannah Kleine-Weber,Simon Schroeder,Nadine Krüger,Tanja Herrler,Sandra Erichsen,Tobias S. Schiergens,Georg Herrler,Nai Huei Wu,Andreas Nitsche,Marcel A. Müller,Christian Drosten,Christian Drosten,Stefan Pöhlmann +13 more
TLDR
It is demonstrated that SARS-CoV-2 uses the SARS -CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming, and it is shown that the sera from convalescent SARS patients cross-neutralized Sars-2-S-driven entry.About:
This article is published in Cell.The article was published on 2020-04-16 and is currently open access. It has received 15362 citations till now. The article focuses on the topics: Proteases.read more
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Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2.
J. Huo,J. Huo,A. Le Bas,A. Le Bas,Reinis R. Ruza,Duyvesteyn Hme.,H. Mikolajek,Tomas Malinauskas,Tiong Kit Tan,Pramila Rijal,Pramila Rijal,Maud Dumoux,Philip N. Ward,Philip N. Ward,Jingshan Ren,D. Zhou,Peter J. Harrison,Peter J. Harrison,Miriam Weckener,Daniel K. Clare,V K Vogirala,Julika Radecke,Lucile Moynié,Yuguang Zhao,Javier Gilbert-Jaramillo,Michael L. Knight,Julia A. Tree,Karen R. Buttigieg,Naomi Coombes,Michael J. Elmore,Miles W. Carroll,Loic Carrique,Shah Pnm.,William James,Alain Townsend,Alain Townsend,David I. Stuart,Raymond J. Owens,Raymond J. Owens,James H. Naismith,James H. Naismith +40 more
TL;DR: Two nanobodies that bind SARS-CoV-2 spike RBD are shown to block interaction with receptor ACE2 and thus neutralize the virus, and have an additive effect with antibody CR3022.
Posted ContentDOI
Non-neural expression of SARS-CoV-2 entry genes in the olfactory epithelium suggests mechanisms underlying anosmia in COVID-19 patients
TL;DR: This article analyzed bulk and single cell RNA-Seq datasets to identify cell types in the olfactory epithelium that express molecules that mediate infection by SARS-CoV-2 (CoV2), the causal agent in COVID-19.
Journal ArticleDOI
Immunity, endothelial injury and complement-induced coagulopathy in COVID-19.
Luca Perico,Ariela Benigni,Federica Casiraghi,Lisa F. P. Ng,Lisa F. P. Ng,Laurent Rénia,Giuseppe Remuzzi,Giuseppe Remuzzi +7 more
TL;DR: Current understanding of the pathogenic mechanisms involved in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and the progression of coronav virus disease 2019 (COVID-19) is described, focusing on the immunological hyper-response and the induction of widespread endothelial damage, complement-associated blood clotting and systemic microangiopathy, as well as the effects of these processes on the kidney.
Journal ArticleDOI
SARS-CoV-2 Omicron variant replication in human bronchus and lung ex vivo
Kenrie P Y Hui,John C. W. Ho,Man Chun Cheung,Ka Chun Ng,Rachel Hiu Ha Ching,Ka-ling Lai,T. Kam,Haogao Gu,Ko-Yung Sit,M. K. Hsin,Timmy W.K. Au,Leo L. M. Poon,Malik Peiris,John M. Nicholls,Michael C. W. Chan +14 more
TL;DR: In this paper , the authors compared the replication competence and cellular tropism of the wild-type SARS-CoV-2 variants of concern with progressively increased transmissibility between humans, and found that Omicron is more dependent on TMPRSS2 and cathepsins for infection.
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SARS-CoV-2 Omicron variant: Antibody evasion and cryo-EM structure of spike protein–ACE2 complex
Dhiraj Mannar,James W. Saville,Xing Zhu,Shanti Swaroop Srivastava,Alison M. Berezuk,Katharine Tuttle,Ana Citlali Márquez,Inna Sekirov,Sriram Subramaniam +8 more
TL;DR: Cryo-EM analysis of the Omicron spike protein reveals how ACE2 binding occurs despite high mutational escape from antibodies, and rationalizes the evasion of antibodies elicited by previous vaccination or infection.
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TL;DR: The findings are consistent with person-to-person transmission of this novel coronavirus in hospital and family settings, and the reports of infected travellers in other geographical regions.
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