SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor
Markus Hoffmann,Hannah Kleine-Weber,Simon Schroeder,Nadine Krüger,Tanja Herrler,Sandra Erichsen,Tobias S. Schiergens,Georg Herrler,Nai Huei Wu,Andreas Nitsche,Marcel A. Müller,Christian Drosten,Christian Drosten,Stefan Pöhlmann +13 more
TLDR
It is demonstrated that SARS-CoV-2 uses the SARS -CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming, and it is shown that the sera from convalescent SARS patients cross-neutralized Sars-2-S-driven entry.About:
This article is published in Cell.The article was published on 2020-04-16 and is currently open access. It has received 15362 citations till now. The article focuses on the topics: Proteases.read more
Citations
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Potent Neutralizing Antibodies against SARS-CoV-2 Identified by High-Throughput Single-Cell Sequencing of Convalescent Patients' B Cells.
Y Cao,Bin Su,Xianghua Guo,Wenjie Sun,Yong-Qiang Deng,Linlin Bao,Qinyu Zhu,Xu Zhang,Yinghui Zheng,Chenyang Geng,Xiaoran Chai,Runsheng He,Xiaofeng Li,Qi Lv,Hua Zhu,Wei Deng,Yanfeng Xu,Yanjun Wang,Luxin Qiao,Yafang Tan,Liyang Song,Guopeng Wang,Xiao-Xia Du,Ning Gao,Jiangning Liu,Junyu Xiao,Xiao-Dong Su,Zongmin Du,Yingmei Feng,Chuan Qin,Cheng-Feng Qin,Ronghua Jin,X. Sunney Xie +32 more
TL;DR: It is shown that human neutralizing antibodies could be efficiently discovered by high-throughput single B-cell sequencing in response to pandemic infectious diseases.
Journal ArticleDOI
COVID-19 and the cardiovascular system: implications for risk assessment, diagnosis, and treatment options.
Tomasz J. Guzik,Tomasz J. Guzik,Saidi A Mohiddin,Anthony Dimarco,Vimal Patel,K Savvatis,Federica M. Marelli-Berg,Meena S. Madhur,Maciej Tomaszewski,Pasquale Maffia,Pasquale Maffia,Fulvio D'Acquisto,Stuart A. Nicklin,Ali J. Marian,Ryszard Nosalski,Ryszard Nosalski,Eleanor Murray,Bartłomiej Guzik,Colin Berry,Rhian M. Touyz,Reinhold Kreutz,Dao Wen Wang,David Bhella,Orlando Sagliocco,Filippo Crea,E. Thomson,Iain B. McInnes +26 more
TL;DR: While ACE2 is essential for viral invasion, there is no evidence that ACE inhibitors or angiotensin receptor blockers (ARBs) worsen prognosis, Hence, patients should not discontinue their use.
Journal ArticleDOI
Mild or Moderate Covid-19.
TL;DR: Covid-19 (the illness caused by SARS-CoV-2) has a range of clinical manifestations, including cough, fever, malaise, myalgias, gastrointestinal symptom...
Journal ArticleDOI
Cardiovascular Disease, Drug Therapy, and Mortality in Covid-19.
TL;DR: Previous observations suggesting that underlying cardiovascular disease is associated with an increased risk of in-hospital death among patients hospitalized with Covid-19 were confirmed, and the results did not confirm previous concerns regarding a potential harmful association of ACE inhibitors or ARBs in this clinical context.
Journal ArticleDOI
Mapping Neutralizing and Immunodominant Sites on the SARS-CoV-2 Spike Receptor-Binding Domain by Structure-Guided High-Resolution Serology.
Luca Piccoli,Young-Jun Park,M. Alejandra Tortorici,M. Alejandra Tortorici,Nadine Czudnochowski,Alexandra C. Walls,Martina Beltramello,Chiara Silacci-Fregni,Dora Pinto,Laura E. Rosen,John E. Bowen,Oliver J. Acton,Stefano Jaconi,Barbara Guarino,Andrea Minola,Fabrizia Zatta,Nicole Sprugasci,Jessica Bassi,Alessia Peter,Anna De Marco,Jay C. Nix,Federico Mele,Sandra Jovic,Blanca Fernandez Rodriguez,Sneha V. Gupta,Feng Jin,Giovanni Piumatti,Giovanni Piumatti,Giorgia Lo Presti,Alessandra Franzetti Pellanda,Maira Biggiogero,Maciej Tarkowski,Matteo Samuele Pizzuto,Elisabetta Cameroni,Colin Havenar-Daughton,Megan Smithey,David K. Hong,Valentino Lepori,Emiliano Albanese,Alessandro Ceschi,Alessandro Ceschi,Enos Bernasconi,Luigia Elzi,Paolo Ferrari,Christian Garzoni,Agostino Riva,G. Snell,Federica Sallusto,Katja Fink,Herbert W. Virgin,Antonio Lanzavecchia,Davide Corti,David Veesler +52 more
TL;DR: It is found that both the magnitude of Ab responses to SARS-CoV-2 spike (S) and nucleoprotein and nAb titers correlate with clinical scores, and the immunodominance of the receptor-binding motif will guide the design of COVID-19 vaccines and therapeutics.
References
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Jasper Fuk-Woo Chan,Shuofeng Yuan,Kin-Hang Kok,Kelvin K. W. To,Kelvin K. W. To,Hin Chu,Jin Yang,Fanfan Xing,Jieling Liu,Cyril C. Y. Yip,Rosana W.S. Poon,Hoi Wah Tsoi,Simon Kam Fai Lo,Kwok-Hung Chan,Vincent Kwok-Man Poon,Wan Mui Chan,Jonathan Daniel Ip,Jian Piao Cai,Vincent C.C. Cheng,Honglin Chen,Honglin Chen,Christopher K.M. Hui,Kwok-Yung Yuen +22 more
TL;DR: The findings are consistent with person-to-person transmission of this novel coronavirus in hospital and family settings, and the reports of infected travellers in other geographical regions.
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