SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor
Markus Hoffmann,Hannah Kleine-Weber,Simon Schroeder,Nadine Krüger,Tanja Herrler,Sandra Erichsen,Tobias S. Schiergens,Georg Herrler,Nai Huei Wu,Andreas Nitsche,Marcel A. Müller,Christian Drosten,Christian Drosten,Stefan Pöhlmann +13 more
TLDR
It is demonstrated that SARS-CoV-2 uses the SARS -CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming, and it is shown that the sera from convalescent SARS patients cross-neutralized Sars-2-S-driven entry.About:
This article is published in Cell.The article was published on 2020-04-16 and is currently open access. It has received 15362 citations till now. The article focuses on the topics: Proteases.read more
Citations
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Airways Expression of SARS-CoV-2 Receptor, ACE2, and TMPRSS2 Is Lower in Children Than Adults and Increases with Smoking and COPD
Narjes Saheb Sharif-Askari,Fatemeh Saheb Sharif-Askari,Mashael Alabed,Mohamed Hani Temsah,Saba Al Heialy,Saba Al Heialy,Qutayba Hamid,Qutayba Hamid,Rabih Halwani,Rabih Halwani +9 more
TL;DR: The results suggest that the observed difference in COVID-19 severity between children and adults could, in part, be attributed to the difference in ACE2 and TMPRSS2 airways tissue expression levels.
Journal ArticleDOI
ORF3a of the COVID-19 virus SARS-CoV-2 blocks HOPS complex-mediated assembly of the SNARE complex required for autolysosome formation.
Guangyan Miao,Hongyu Zhao,Yan Li,Mingming Ji,Yong Chen,Yi Shi,Yuhai Bi,Pei-Hui Wang,Hong Zhang +8 more
TL;DR: The study reveals a mechanism by which SARS-CoV-2 evades lysosomal destruction and provides insights for developing new strategies to treat COVID-19 and Surprisingly, ORF3a from the SARS virus SARS -CoV fails to interact with HOPS or block autophagy.
Journal ArticleDOI
Does SARS-Cov-2 invade the brain? Translational lessons from animal models.
TL;DR: The current knowledge from models of previous CoV infections and their potential relevance to COVID‐19 are described and discussed.
Journal ArticleDOI
Structurally Resolved SARS-CoV-2 Antibody Shows High Efficacy in Severely Infected Hamsters and Provides a Potent Cocktail Pairing Strategy.
Shuo Du,Y Cao,Qinyu Zhu,Pin Yu,Feifei Qi,Guopeng Wang,Xiao-Xia Du,Linlin Bao,Wei Deng,Hua Zhu,Jiangning Liu,Jianhui Nie,Yinghui Zheng,Haoyu Liang,Ruixue Liu,Shuran Gong,Hua Xu,Ayijiang Yisimayi,Qi Lv,Bo Wang,Runsheng He,Yunlin Han,Wenjie Zhao,Yali Bai,Yajin Qu,Xiang Gao,Chenggong Ji,Qisheng Wang,Ning Gao,Weijin Huang,Youchun Wang,X. Sunney Xie,Xiao-Dong Su,Junyu Xiao,Chuan Qin +34 more
TL;DR: The results rationalized a new RBD epitope that leads to high neutralization potency and demonstrated BD-368-2’s therapeutic potential in treating COVID-19, a SARS-CoV-2 pseudovirus at pM level and rescues mutation-induced neutralization escapes.
Journal ArticleDOI
Multi-organ proteomic landscape of COVID-19 autopsies.
Xiu Nie,Liujia Qian,Liujia Qian,Rui Sun,Rui Sun,Bo Huang,Xiaochuan Dong,Qi Xiao,Qi Xiao,Qiushi Zhang,Tian Lu,Tian Lu,Liang Yue,Liang Yue,Shuo Chen,Xiang Li,Yaoting Sun,Yaoting Sun,Lu Li,Lu Li,Luang Xu,Luang Xu,Yan Li,Ming Yang,Zhangzhi Xue,Zhangzhi Xue,Shuang Liang,Shuang Liang,Xuan Ding,Xuan Ding,Chunhui Yuan,Chunhui Yuan,Li Peng,Wei Liu,Wei Liu,Xiao Yi,Xiao Yi,Mengge Lyu,Mengge Lyu,Guixiang Xiao,Xia Xu,Weigang Ge,Jiale He,Jiale He,Jun Fan,Junhua Wu,Meng Luo,Xiaona Chang,Huaxiong Pan,Xue Cai,Xue Cai,Junjie Zhou,Jing Yu,Jing Yu,Huanhuan Gao,Huanhuan Gao,Mingxing Xie,Sihua Wang,Guan Ruan,Guan Ruan,Hao Chen,Hua Su,Heng Mei,Danju Luo,Dashi Zhao,Fei Xu,Yi Zhu,Yi Zhu,Jiahong Xia,Yu Hu,Tiannan Guo,Tiannan Guo +71 more
TL;DR: In this article, the authors report a proteomic analysis of 144 autopsy samples from seven organs in 19 COVID-19 patients and quantified 11,394 proteins in these samples, in which 5,336 were perturbed in the COVID19 patients compared to controls.
References
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TL;DR: The findings are consistent with person-to-person transmission of this novel coronavirus in hospital and family settings, and the reports of infected travellers in other geographical regions.
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