SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor
Markus Hoffmann,Hannah Kleine-Weber,Simon Schroeder,Nadine Krüger,Tanja Herrler,Sandra Erichsen,Tobias S. Schiergens,Georg Herrler,Nai Huei Wu,Andreas Nitsche,Marcel A. Müller,Christian Drosten,Christian Drosten,Stefan Pöhlmann +13 more
TLDR
It is demonstrated that SARS-CoV-2 uses the SARS -CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming, and it is shown that the sera from convalescent SARS patients cross-neutralized Sars-2-S-driven entry.About:
This article is published in Cell.The article was published on 2020-04-16 and is currently open access. It has received 15362 citations till now. The article focuses on the topics: Proteases.read more
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SARS-CoV-2 immune evasion by the B.1.427/B.1.429 variant of concern.
Matthew McCallum,Jessica Bassi,Anna De Marco,Alexander Chen,Alexandra C. Walls,Julia di Iulio,M. Alejandra Tortorici,Mary-Jane Navarro,Chiara Silacci-Fregni,Christian Saliba,Kaitlin R. Sprouse,Maria De Agostini,Dora Pinto,Katja Culap,Siro Bianchi,Stefano Jaconi,Elisabetta Cameroni,John E. Bowen,Sasha W Tilles,Matteo Samuele Pizzuto,Sonja Bernasconi Guastalla,Giovanni Bona,Alessandra Franzetti Pellanda,Christian Garzoni,Wesley C. Van Voorhis,Laura E. Rosen,Gyorgy Snell,Amalio Telenti,Herbert W. Virgin,Luca Piccoli,Davide Corti,David Veesler +31 more
TL;DR: In this paper, a novel variant of concern (VOC) named CAL.20C (B.1.427/B. 1.429), which was originally detected in California, carries spike glycoprotein mutations S13I in the signal peptide, W152C in the N-terminal domain (NTD), and L452R in the receptor-binding domain (RBD).
Journal ArticleDOI
Immunological dysfunction persists for 8 months following initial mild-to-moderate SARS-CoV-2 infection
TL;DR: Phetsouphanh et al. as mentioned in this paper showed that individuals with long COVID have persistent activation of the innate and adaptive immune system at 8 months after infection and define a set of analytes associated with long CoVID with 78.5-81.6% accuracy.
Journal ArticleDOI
Free fatty acid binding pocket in the locked structure of SARS-CoV-2 spike protein.
Christine Toelzer,Kapil Gupta,K.N. Sathish Yadav,Ufuk Borucu,Andrew D. Davidson,Maia Kavanagh Williamson,Deborah K. Shoemark,Frederic Garzoni,Oskar Staufer,Rachel Milligan,Julien Capin,Adrian J. Mulholland,Joachim P. Spatz,Daniel J. Fitzgerald,Imre Berger,Christiane Schaffitzel +15 more
TL;DR: The 2.85-angstrom cryo–electron microscopy structure of SARS-CoV-2 spike (S) glycoprotein reveals that the receptor binding domains tightly bind the essential free fatty acid linoleic acid (LA) in three composite binding pockets.
Journal ArticleDOI
SARS-CoV-2 infects and replicates in cells of the human endocrine and exocrine pancreas.
Janis A. Müller,Rüdiger Groß,Carina Conzelmann,Jana Krüger,Uta Merle,Johannes Steinhart,Tatjana Weil,Lennart Koepke,Caterina Prelli Bozzo,Clarissa Read,Giorgio Fois,Tim Eiseler,Julia Gehrmann,Joanne van Vuuren,Isabel M Wessbecher,Manfred Frick,Ivan G. Costa,Markus Breunig,Beate Grüner,Lynn Peters,Michael Schuster,Stefan Liebau,Thomas Seufferlein,Steffen Stenger,Albrecht Stenzinger,Patrick E. MacDonald,Frank Kirchhoff,Konstantin M. J. Sparrer,Paul Walther,Heiko Lickert,Thomas F. E. Barth,Martin Wagner,Jan Münch,Sandra Heller,Alexander Kleger +34 more
TL;DR: In this paper, the authors identify the human pancreas as a target of SARS-CoV-2 infection and suggest that β-cell infection could contribute to the metabolic dysregulation observed in patients with COVID-19.
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Interactions of coronaviruses with ACE2, angiotensin II, and RAS inhibitors-lessons from available evidence and insights into COVID-19.
Hisashi Kai,Mamiko Kai +1 more
TL;DR: There is no clinical or experimental evidence supporting that ARBs and ACEIs either augment the susceptibility to SARS-CoV-2 or aggravate the severity and outcomes of COVID-19 at present.
References
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Journal ArticleDOI
Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China
Chaolin Huang,Yeming Wang,Xingwang Li,Lili Ren,Jianping Zhao,Yi Hu,Li Zhang,Guohui Fan,Jiuyang Xu,Xiaoying Gu,Zhenshun Cheng,Ting Yu,Jia'an Xia,Yuan Wei,Wenjuan Wu,Xuelei Xie,Wen Yin,Li Hui,Min Liu,Yan Xiao,Hong Gao,Li Guo,Jungang Xie,Guang-Fa Wang,Rongmeng Jiang,Zhancheng Gao,Qi Jin,Jianwei Wang,Bin Cao +28 more
TL;DR: The epidemiological, clinical, laboratory, and radiological characteristics and treatment and clinical outcomes of patients with laboratory-confirmed 2019-nCoV infection in Wuhan, China, were reported.
Journal ArticleDOI
MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms.
TL;DR: The Molecular Evolutionary Genetics Analysis (Mega) software implements many analytical methods and tools for phylogenomics and phylomedicine and has additionally been upgraded to use multiple computing cores for many molecular evolutionary analyses.
Journal ArticleDOI
A Novel Coronavirus from Patients with Pneumonia in China, 2019.
Na Zhu,Dingyu Zhang,Wenling Wang,Xingwang Li,Bo Yang,Jingdong Song,Xiang Zhao,Baoying Huang,Weifeng Shi,Roujian Lu,Peihua Niu,Faxian Zhan,Xuejun Ma,Dayan Wang,Wenbo Xu,Wenbo Xu,Guizhen Wu,George F. Gao,Wenjie Tan +18 more
TL;DR: Human airway epithelial cells were used to isolate a novel coronavirus, named 2019-nCoV, which formed a clade within the subgenus sarbecovirus, Orthocoronavirinae subfamily, which is the seventh member of the family of coronaviruses that infect humans.
Journal ArticleDOI
A pneumonia outbreak associated with a new coronavirus of probable bat origin
Peng Zhou,Xing-Lou Yang,Xian Guang Wang,Ben Hu,Lei Zhang,Wei Zhang,Hao Rui Si,Yan Zhu,Bei Li,Chao Lin Huang,Hui-Dong Chen,Jing Chen,Yun Luo,Hua Guo,Ren Di Jiang,Meiqin Liu,Ying Chen,Xu Rui Shen,Xi Wang,Xiao Shuang Zheng,Kai Zhao,Quanjiao Chen,Fei Deng,Lin Lin Liu,Bing Yan,Fa Xian Zhan,Yan-Yi Wang,Gengfu Xiao,Zhengli Shi +28 more
TL;DR: Identification and characterization of a new coronavirus (2019-nCoV), which caused an epidemic of acute respiratory syndrome in humans in Wuhan, China, and it is shown that this virus belongs to the species of SARSr-CoV, indicates that the virus is related to a bat coronav virus.
Journal ArticleDOI
A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster.
Jasper Fuk-Woo Chan,Shuofeng Yuan,Kin-Hang Kok,Kelvin K. W. To,Kelvin K. W. To,Hin Chu,Jin Yang,Fanfan Xing,Jieling Liu,Cyril C. Y. Yip,Rosana W.S. Poon,Hoi Wah Tsoi,Simon Kam Fai Lo,Kwok-Hung Chan,Vincent Kwok-Man Poon,Wan Mui Chan,Jonathan Daniel Ip,Jian Piao Cai,Vincent C.C. Cheng,Honglin Chen,Honglin Chen,Christopher K.M. Hui,Kwok-Yung Yuen +22 more
TL;DR: The findings are consistent with person-to-person transmission of this novel coronavirus in hospital and family settings, and the reports of infected travellers in other geographical regions.
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