SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor
Markus Hoffmann,Hannah Kleine-Weber,Simon Schroeder,Nadine Krüger,Tanja Herrler,Sandra Erichsen,Tobias S. Schiergens,Georg Herrler,Nai Huei Wu,Andreas Nitsche,Marcel A. Müller,Christian Drosten,Christian Drosten,Stefan Pöhlmann +13 more
TLDR
It is demonstrated that SARS-CoV-2 uses the SARS -CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming, and it is shown that the sera from convalescent SARS patients cross-neutralized Sars-2-S-driven entry.About:
This article is published in Cell.The article was published on 2020-04-16 and is currently open access. It has received 15362 citations till now. The article focuses on the topics: Proteases.read more
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Single-cell multiomic profiling of human lungs reveals cell-type-specific and age-dynamic control of SARS-CoV2 host genes.
Allen Wang,Joshua Chiou,Olivier Poirion,Justin Buchanan,Michael J Valdez,Jamie M. Verheyden,Xiaomeng Hou,Parul Kudtarkar,Sharvari Narendra,Jacklyn M Newsome,Minzhe Guo,Minzhe Guo,Dina A. Faddah,Kai Zhang,Randee E. Young,Randee E. Young,Justinn Barr,Eniko Sajti,Ravi S. Misra,Heidie Huyck,Lisa Rogers,Cory Poole,JA Whitsett,JA Whitsett,Gloria S. Pryhuber,Yan Xu,Yan Xu,Kyle J. Gaulton,Sebastian Preissl,Xin Sun +29 more
TL;DR: These datasets will facilitate interpretation of risk loci for lung diseases and provide insight into regulatory logic underlying genes implicated in COVID-19 in individual lung cell types across age.
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Broad and Differential Animal Angiotensin-Converting Enzyme 2 Receptor Usage by SARS-CoV-2.
Xuesen Zhao,Danying Chen,Robert Szabla,Mei Zheng,Guoli Li,Pengcheng Du,Shuangli Zheng,Xinglin Li,Chuan Song,Rui Li,Ju-Tao Guo,Murray S. Junop,Hui Zeng,Hanxin Lin +13 more
TL;DR: Examination of receptor activity of 14 ACE2 orthologs found that wild-type and mutant SARS-CoV-2 lacking the furin cleavage site in S protein could utilize ACE2 from a broad range of animal species to enter host cells and have important implications for understanding potential natural reservoirs, zoonotic transmission, human-to-animal transmission, and use of animal models.
Journal ArticleDOI
SARS-CoV-2-neutralising monoclonal antibodies for treatment of COVID-19
Nina Kreuzberger,Caroline Hirsch,Khai Li Chai,Vanessa Piechotta,Sarah J Valk,Lise J Estcourt,Susanne Salomon,Eve Tomlinson,Ina Monsef,Erica M. Wood,Cynthia So-Osman,David Roberts,Zoe McQuilten,Nicole Skoetz +13 more
TL;DR: This is a protocol for a Cochrane Review to assess whether SARS-CoV-2-neutralising mAbs are effective and safe for treating patients with COVID-19 in comparison to an active comparator, placebo or no intervention.
Journal ArticleDOI
In Silico Exploration of the Molecular Mechanism of Clinically Oriented Drugs for Possibly Inhibiting SARS-CoV-2's Main Protease.
TL;DR: An important ligand binding mechanism of the Mpro is revealed, demonstrating that the binding stability of a ligand inside the M Pro pocket can be significantly improved if part of the ligand occupies its so-called “anchor” site.
Posted ContentDOI
The SARS-CoV-2 receptor-binding domain elicits a potent neutralizing response without antibody-dependent enhancement
Brian D. Quinlan,Huihui Mou,Lizhou Zhang,Yan Guo,Wenhui He,Amrita Ojha,Mark S. Parcells,Guangxiang Luo,Wenhui Li,Guocai Zhong,Guocai Zhong,Hyeryun Choe,Michael Farzan +12 more
TL;DR: The data suggest that an RBD-based vaccine for SARS-CoV-2 could be safe and effective, and that anti-sera from immunized animals did not mediate antibody-dependent enhancement of S-protein-mediated entry under conditions in which Zika virus ADE was readily observed.
References
More filters
Journal ArticleDOI
Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China
Chaolin Huang,Yeming Wang,Xingwang Li,Lili Ren,Jianping Zhao,Yi Hu,Li Zhang,Guohui Fan,Jiuyang Xu,Xiaoying Gu,Zhenshun Cheng,Ting Yu,Jia'an Xia,Yuan Wei,Wenjuan Wu,Xuelei Xie,Wen Yin,Li Hui,Min Liu,Yan Xiao,Hong Gao,Li Guo,Jungang Xie,Guang-Fa Wang,Rongmeng Jiang,Zhancheng Gao,Qi Jin,Jianwei Wang,Bin Cao +28 more
TL;DR: The epidemiological, clinical, laboratory, and radiological characteristics and treatment and clinical outcomes of patients with laboratory-confirmed 2019-nCoV infection in Wuhan, China, were reported.
Journal ArticleDOI
MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms.
TL;DR: The Molecular Evolutionary Genetics Analysis (Mega) software implements many analytical methods and tools for phylogenomics and phylomedicine and has additionally been upgraded to use multiple computing cores for many molecular evolutionary analyses.
Journal ArticleDOI
A Novel Coronavirus from Patients with Pneumonia in China, 2019.
Na Zhu,Dingyu Zhang,Wenling Wang,Xingwang Li,Bo Yang,Jingdong Song,Xiang Zhao,Baoying Huang,Weifeng Shi,Roujian Lu,Peihua Niu,Faxian Zhan,Xuejun Ma,Dayan Wang,Wenbo Xu,Wenbo Xu,Guizhen Wu,George F. Gao,Wenjie Tan +18 more
TL;DR: Human airway epithelial cells were used to isolate a novel coronavirus, named 2019-nCoV, which formed a clade within the subgenus sarbecovirus, Orthocoronavirinae subfamily, which is the seventh member of the family of coronaviruses that infect humans.
Journal ArticleDOI
A pneumonia outbreak associated with a new coronavirus of probable bat origin
Peng Zhou,Xing-Lou Yang,Xian Guang Wang,Ben Hu,Lei Zhang,Wei Zhang,Hao Rui Si,Yan Zhu,Bei Li,Chao Lin Huang,Hui-Dong Chen,Jing Chen,Yun Luo,Hua Guo,Ren Di Jiang,Meiqin Liu,Ying Chen,Xu Rui Shen,Xi Wang,Xiao Shuang Zheng,Kai Zhao,Quanjiao Chen,Fei Deng,Lin Lin Liu,Bing Yan,Fa Xian Zhan,Yan-Yi Wang,Gengfu Xiao,Zhengli Shi +28 more
TL;DR: Identification and characterization of a new coronavirus (2019-nCoV), which caused an epidemic of acute respiratory syndrome in humans in Wuhan, China, and it is shown that this virus belongs to the species of SARSr-CoV, indicates that the virus is related to a bat coronav virus.
Journal ArticleDOI
A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster.
Jasper Fuk-Woo Chan,Shuofeng Yuan,Kin-Hang Kok,Kelvin K. W. To,Kelvin K. W. To,Hin Chu,Jin Yang,Fanfan Xing,Jieling Liu,Cyril C. Y. Yip,Rosana W.S. Poon,Hoi Wah Tsoi,Simon Kam Fai Lo,Kwok-Hung Chan,Vincent Kwok-Man Poon,Wan Mui Chan,Jonathan Daniel Ip,Jian Piao Cai,Vincent C.C. Cheng,Honglin Chen,Honglin Chen,Christopher K.M. Hui,Kwok-Yung Yuen +22 more
TL;DR: The findings are consistent with person-to-person transmission of this novel coronavirus in hospital and family settings, and the reports of infected travellers in other geographical regions.
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