SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor
Markus Hoffmann,Hannah Kleine-Weber,Simon Schroeder,Nadine Krüger,Tanja Herrler,Sandra Erichsen,Tobias S. Schiergens,Georg Herrler,Nai Huei Wu,Andreas Nitsche,Marcel A. Müller,Christian Drosten,Christian Drosten,Stefan Pöhlmann +13 more
TLDR
It is demonstrated that SARS-CoV-2 uses the SARS -CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming, and it is shown that the sera from convalescent SARS patients cross-neutralized Sars-2-S-driven entry.About:
This article is published in Cell.The article was published on 2020-04-16 and is currently open access. It has received 15362 citations till now. The article focuses on the topics: Proteases.read more
Citations
More filters
Journal ArticleDOI
Broad neutralization of SARS-related viruses by human monoclonal antibodies.
Anna Z. Wec,Daniel Wrapp,Andrew S. Herbert,Daniel P. Maurer,Denise Haslwanter,Mrunal Sakharkar,Rohit K. Jangra,M. Eugenia Dieterle,Asparouh Lilov,Deli Huang,Longping V. Tse,Nicole V. Johnson,Ching-Lin Hsieh,Nianshuang Wang,Juergen Hermann Nett,Elizabeth Champney,Irina Burnina,Michael G. Brown,Shu Lin,Melanie Sinclair,Carl Hirschie Johnson,Sarat Pudi,Robert H. Bortz,Ariel S. Wirchnianski,Ethan Laudermilch,Catalina Florez,J. Maximilian Fels,Cecilia M. O’Brien,Barney S. Graham,David Nemazee,Dennis R. Burton,Ralph S. Baric,James E. Voss,Kartik Chandran,John M. Dye,Jason S. McLellan,Laura M. Walker +36 more
TL;DR: Hundreds of antibodies against the viral spike protein of SARS-CoV-2 are isolated from the memory B cells of a survivor of the 2003 outbreak and reveal a target for the rational design of pan-sarbecovirus vaccines.
Journal ArticleDOI
Naturally enhanced neutralizing breadth against SARS-CoV-2 one year after infection.
Zijun Wang,Frauke Muecksch,Dennis Schaefer-Babajew,Shlomo Finkin,Charlotte Viant,Christian Gaebler,Hans Heinrich Hoffmann,Christopher O. Barnes,Melissa Cipolla,Victor A. Ramos,Thiago Y. Oliveira,Alice Cho,Fabian Schmidt,Justin Da Silva,Eva Bednarski,Lauren C. Aguado,Jim Yee,Mridushi Daga,Martina Turroja,Katrina G. Millard,Mila Jankovic,Anna Gazumyan,Anna Gazumyan,Zhen Zhao,Charles M. Rice,Paul D. Bieniasz,Paul D. Bieniasz,Marina Caskey,Theodora Hatziioannou,Michel C. Nussenzweig,Michel C. Nussenzweig +30 more
TL;DR: In the absence of vaccination, antibody reactivity to the receptor binding domain (RBD) of SARS-CoV-2, neutralizing activity and the number of RBD-specific memory B cells remain relatively stable between 6 and 12 months after infection.
Journal ArticleDOI
A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids.
Liuliu Yang,Yuling Han,Benjamin E. Nilsson-Payant,Vikas Gupta,Pengfei Wang,Xiaohua Duan,Xiaohua Duan,Xuming Tang,Jiajun Zhu,Zeping Zhao,Fabrice Jaffré,Tuo Zhang,Tae Wan Kim,Oliver Harschnitz,David Redmond,Sean Houghton,Chengyang Liu,Ali Naji,Gabriele Ciceri,Sudha R Guttikonda,Yaron Bram,Duc-Huy T. Nguyen,Michele Cioffi,Vasuretha Chandar,Daisy A. Hoagland,Yaoxing Huang,Jenny Xiang,Hui Wang,Hui Wang,David Lyden,Alain C. Borczuk,Huanhuan Joyce Chen,Lorenz Studer,Fong Cheng Pan,David D. Ho,Benjamin R. tenOever,Todd Evans,Robert E. Schwartz,Shuibing Chen +38 more
TL;DR: It is found that human pancreatic beta cells and liver organoids are highly permissive to SARS-CoV-2 infection, further validated using adult primary human islets and adult hepatocyte and cholangiocyte organoids.
Journal ArticleDOI
Antibody-dependent enhancement and SARS-CoV-2 vaccines and therapies.
TL;DR: Key ADE mechanisms are described and mitigation strategies for SARS-CoV-2 vaccines and therapies in development are discussed and recently published data is outlined to evaluate the risks and opportunities for antibody-based protection against Sars-Cov-2.
Journal ArticleDOI
Myocarditis and inflammatory cardiomyopathy: current evidence and future directions.
Carsten Tschöpe,Enrico Ammirati,Biykem Bozkurt,Biykem Bozkurt,Alida L.P. Caforio,Leslie T. Cooper,Stephan B. Felix,Joshua M. Hare,Bettina Heidecker,Stephane Heymans,Stephane Heymans,Norbert Hubner,Sebastian Kelle,Karin Klingel,Henrike Maatz,Abdul Shokor Parwani,Frank Spillmann,Randall C. Starling,Hiroyuki Tsutsui,Petar M. Seferovic,Sophie Van Linthout +20 more
TL;DR: Improved standardization of available invasive and noninvasive diagnostic tools and a consensus on their specific use are needed to allow specific diagnosis and stratification of patient cohorts for the implementation of aetiology-based therapies.
References
More filters
Journal ArticleDOI
Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China
Chaolin Huang,Yeming Wang,Xingwang Li,Lili Ren,Jianping Zhao,Yi Hu,Li Zhang,Guohui Fan,Jiuyang Xu,Xiaoying Gu,Zhenshun Cheng,Ting Yu,Jia'an Xia,Yuan Wei,Wenjuan Wu,Xuelei Xie,Wen Yin,Li Hui,Min Liu,Yan Xiao,Hong Gao,Li Guo,Jungang Xie,Guang-Fa Wang,Rongmeng Jiang,Zhancheng Gao,Qi Jin,Jianwei Wang,Bin Cao +28 more
TL;DR: The epidemiological, clinical, laboratory, and radiological characteristics and treatment and clinical outcomes of patients with laboratory-confirmed 2019-nCoV infection in Wuhan, China, were reported.
Journal ArticleDOI
MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms.
TL;DR: The Molecular Evolutionary Genetics Analysis (Mega) software implements many analytical methods and tools for phylogenomics and phylomedicine and has additionally been upgraded to use multiple computing cores for many molecular evolutionary analyses.
Journal ArticleDOI
A Novel Coronavirus from Patients with Pneumonia in China, 2019.
Na Zhu,Dingyu Zhang,Wenling Wang,Xingwang Li,Bo Yang,Jingdong Song,Xiang Zhao,Baoying Huang,Weifeng Shi,Roujian Lu,Peihua Niu,Faxian Zhan,Xuejun Ma,Dayan Wang,Wenbo Xu,Wenbo Xu,Guizhen Wu,George F. Gao,Wenjie Tan +18 more
TL;DR: Human airway epithelial cells were used to isolate a novel coronavirus, named 2019-nCoV, which formed a clade within the subgenus sarbecovirus, Orthocoronavirinae subfamily, which is the seventh member of the family of coronaviruses that infect humans.
Journal ArticleDOI
A pneumonia outbreak associated with a new coronavirus of probable bat origin
Peng Zhou,Xing-Lou Yang,Xian Guang Wang,Ben Hu,Lei Zhang,Wei Zhang,Hao Rui Si,Yan Zhu,Bei Li,Chao Lin Huang,Hui-Dong Chen,Jing Chen,Yun Luo,Hua Guo,Ren Di Jiang,Meiqin Liu,Ying Chen,Xu Rui Shen,Xi Wang,Xiao Shuang Zheng,Kai Zhao,Quanjiao Chen,Fei Deng,Lin Lin Liu,Bing Yan,Fa Xian Zhan,Yan-Yi Wang,Gengfu Xiao,Zhengli Shi +28 more
TL;DR: Identification and characterization of a new coronavirus (2019-nCoV), which caused an epidemic of acute respiratory syndrome in humans in Wuhan, China, and it is shown that this virus belongs to the species of SARSr-CoV, indicates that the virus is related to a bat coronav virus.
Journal ArticleDOI
A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster.
Jasper Fuk-Woo Chan,Shuofeng Yuan,Kin-Hang Kok,Kelvin K. W. To,Kelvin K. W. To,Hin Chu,Jin Yang,Fanfan Xing,Jieling Liu,Cyril C. Y. Yip,Rosana W.S. Poon,Hoi Wah Tsoi,Simon Kam Fai Lo,Kwok-Hung Chan,Vincent Kwok-Man Poon,Wan Mui Chan,Jonathan Daniel Ip,Jian Piao Cai,Vincent C.C. Cheng,Honglin Chen,Honglin Chen,Christopher K.M. Hui,Kwok-Yung Yuen +22 more
TL;DR: The findings are consistent with person-to-person transmission of this novel coronavirus in hospital and family settings, and the reports of infected travellers in other geographical regions.
Related Papers (5)
Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China
Chaolin Huang,Yeming Wang,Xingwang Li,Lili Ren,Jianping Zhao,Yi Hu,Li Zhang,Guohui Fan,Jiuyang Xu,Xiaoying Gu,Zhenshun Cheng,Ting Yu,Jia'an Xia,Yuan Wei,Wenjuan Wu,Xuelei Xie,Wen Yin,Li Hui,Min Liu,Yan Xiao,Hong Gao,Li Guo,Jungang Xie,Guang-Fa Wang,Rongmeng Jiang,Zhancheng Gao,Qi Jin,Jianwei Wang,Bin Cao +28 more
A pneumonia outbreak associated with a new coronavirus of probable bat origin
Peng Zhou,Xing-Lou Yang,Xian Guang Wang,Ben Hu,Lei Zhang,Wei Zhang,Hao Rui Si,Yan Zhu,Bei Li,Chao Lin Huang,Hui-Dong Chen,Jing Chen,Yun Luo,Hua Guo,Ren Di Jiang,Meiqin Liu,Ying Chen,Xu Rui Shen,Xi Wang,Xiao Shuang Zheng,Kai Zhao,Quanjiao Chen,Fei Deng,Lin Lin Liu,Bing Yan,Fa Xian Zhan,Yan-Yi Wang,Gengfu Xiao,Zhengli Shi +28 more
Clinical Characteristics of Coronavirus Disease 2019 in China.
Wei-jie Guan,Zhengyi Ni,Yu Hu,Wenhua Liang,Chun-Quan Ou,Jianxing He,Lei Liu,Hong Shan,Chunliang Lei,David S.C. Hui,Bin Du,Lanjuan Li,Guang Zeng,Kowk-Yung Yuen,Ruchong Chen,Chun-Li Tang,Tao Wang,Ping-Yan Chen,Jie Xiang,Shiyue Li,Jinlin Wang,Zi-jing Liang,Yi-xiang Peng,Li Wei,Yong Liu,Ya-hua Hu,Peng Peng,Jian-ming Wang,Ji-yang Liu,Zhong Chen,Gang Li,Zhi-jian Zheng,Shao-qin Qiu,Jie Luo,Chang-jiang Ye,Shao-yong Zhu,Nanshan Zhong +36 more