SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor
Markus Hoffmann,Hannah Kleine-Weber,Simon Schroeder,Nadine Krüger,Tanja Herrler,Sandra Erichsen,Tobias S. Schiergens,Georg Herrler,Nai Huei Wu,Andreas Nitsche,Marcel A. Müller,Christian Drosten,Christian Drosten,Stefan Pöhlmann +13 more
TLDR
It is demonstrated that SARS-CoV-2 uses the SARS -CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming, and it is shown that the sera from convalescent SARS patients cross-neutralized Sars-2-S-driven entry.About:
This article is published in Cell.The article was published on 2020-04-16 and is currently open access. It has received 15362 citations till now. The article focuses on the topics: Proteases.read more
Citations
More filters
Journal ArticleDOI
Virus-induced senescence is a driver and therapeutic target in COVID-19.
Soyoung Lee,Soyoung Lee,Yong Yu,Jakob Trimpert,Fahad Benthani,Mario Mairhofer,Paulina Richter-Pechanska,Emanuel Wyler,Dimitri Belenki,Dimitri Belenki,Sabine Kaltenbrunner,Maria Pammer,Lea Kausche,Theresa C. Firsching,Kristina Dietert,Michael Schotsaert,Carles Martínez-Romero,Gagandeep Singh,Séverine Kunz,Daniela Niemeyer,Riad Ghanem,Helmut J. F. Salzer,Christian Paar,Michael Mülleder,Melissa Uccellini,Melissa Uccellini,Edward Georg Michaelis,Amjad Khan,Andrea Lau,Martin Schönlein,Martin Schönlein,Anna Habringer,Josef Tomasits,Julia Adler,Susanne Kimeswenger,Achim D. Gruber,Wolfram Hoetzenecker,Herta Steinkellner,Bettina Purfürst,Reinhard Motz,Francesco Di Pierro,Bernd Lamprecht,Nikolaus Osterrieder,Nikolaus Osterrieder,Markus Landthaler,Christian Drosten,Adolfo García-Sastre,Rupert Langer,Markus Ralser,Markus Ralser,Roland Eils,Maurice Reimann,Dorothy N. Y. Fan,Clemens A. Schmitt +53 more
TL;DR: In this article, the authors show that SARS-CoV-2 evokes cellular senescence as a primary stress response in infected cells, and suggest that senolytic targeting of virus-infected cells is a treatment option against SARS and perhaps other viral infections.
Journal ArticleDOI
Exploring the SARS-CoV-2 virus-host-drug interactome for drug repurposing.
Sepideh Sadegh,Julian Matschinske,David Blumenthal,Gihanna Galindez,Tim Kacprowski,Markus List,Reza Nasirigerdeh,Mhaned Oubounyt,Andreas Pichlmair,Tim Daniel Rose,Marisol Salgado-Albarrán,Marisol Salgado-Albarrán,Julian Späth,Alexey Stukalov,Nina K. Wenke,Kevin Yuan,Josch K. Pauling,Jan Baumbach,Jan Baumbach +18 more
TL;DR: CoVex as discussed by the authors is an interactive online platform for SARS-CoV-2 host interactome exploration and drug (target) identification, which integrates virus-human protein interactions, human protein-protein interactions, and drug-target interactions.
Journal ArticleDOI
Inhibition of PIKfyve kinase prevents infection by Zaire ebolavirus and SARS-CoV-2.
Yuan Lin Kang,Yi-ying Chou,Paul W. Rothlauf,Paul W. Rothlauf,Zhuoming Liu,Timothy K. Soh,David K. Cureton,David K. Cureton,James Brett Case,Rita E. Chen,Michael S. Diamond,Sean P. J. Whelan,Tom Kirchhausen +12 more
TL;DR: These results define tools for studying the intracellular trafficking of pathogens elicited by inhibition of PIKfyve kinase and suggest the potential for targeting this kinase in developing small-molecule antivirals against SARS-CoV-2.
Journal ArticleDOI
Impaired local intrinsic immunity to SARS-CoV-2 infection in severe COVID-19.
Carly G. K. Ziegler,Vincent N. Miao,Anna H. Owings,Andrew W. Navia,Ying Tang,Joshua D. Bromley,Peter Lotfy,Peter Lotfy,Meredith Sloan,Hannah Laird,Haley B. Williams,Micayla George,Micayla George,Micayla George,Riley S. Drake,Riley S. Drake,Riley S. Drake,Taylor Christian,Adam M. Parker,Campbell B Sindel,Molly W. Burger,Yilianys Pride,Mohammad H. Hasan,George E. Abraham,Michal Senitko,Tanya O. Robinson,Alex K. Shalek,Sarah C. Glover,Bruce H. Horwitz,Bruce H. Horwitz,Jose Ordovas-Montanes +30 more
TL;DR: In this article, the authors performed scRNA-seq on nasopharyngeal swabs from 58 healthy and COVID-19 participants, and identified genes associated with susceptibility, resistance, or infection response.
Journal ArticleDOI
COVID-19-associated gastrointestinal and liver injury: clinical features and potential mechanisms.
Peijie Zhong,Jing Xu,Dong Yang,Yue Shen,Lu Wang,Yun Feng,Chunling Du,Yuanlin Song,Chaomin Wu,Xianglin Hu,Yangbai Sun +10 more
TL;DR: COVID-19-related liver injury may be due to drug-induced liver injury, systemic inflammatory reaction, and hypoxia–ischemia reperfusion injury, and the direct toxic attack of SARS-CoV-2 on the liver is still questionable.
References
More filters
Journal ArticleDOI
Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China
Chaolin Huang,Yeming Wang,Xingwang Li,Lili Ren,Jianping Zhao,Yi Hu,Li Zhang,Guohui Fan,Jiuyang Xu,Xiaoying Gu,Zhenshun Cheng,Ting Yu,Jia'an Xia,Yuan Wei,Wenjuan Wu,Xuelei Xie,Wen Yin,Li Hui,Min Liu,Yan Xiao,Hong Gao,Li Guo,Jungang Xie,Guang-Fa Wang,Rongmeng Jiang,Zhancheng Gao,Qi Jin,Jianwei Wang,Bin Cao +28 more
TL;DR: The epidemiological, clinical, laboratory, and radiological characteristics and treatment and clinical outcomes of patients with laboratory-confirmed 2019-nCoV infection in Wuhan, China, were reported.
Journal ArticleDOI
MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms.
TL;DR: The Molecular Evolutionary Genetics Analysis (Mega) software implements many analytical methods and tools for phylogenomics and phylomedicine and has additionally been upgraded to use multiple computing cores for many molecular evolutionary analyses.
Journal ArticleDOI
A Novel Coronavirus from Patients with Pneumonia in China, 2019.
Na Zhu,Dingyu Zhang,Wenling Wang,Xingwang Li,Bo Yang,Jingdong Song,Xiang Zhao,Baoying Huang,Weifeng Shi,Roujian Lu,Peihua Niu,Faxian Zhan,Xuejun Ma,Dayan Wang,Wenbo Xu,Wenbo Xu,Guizhen Wu,George F. Gao,Wenjie Tan +18 more
TL;DR: Human airway epithelial cells were used to isolate a novel coronavirus, named 2019-nCoV, which formed a clade within the subgenus sarbecovirus, Orthocoronavirinae subfamily, which is the seventh member of the family of coronaviruses that infect humans.
Journal ArticleDOI
A pneumonia outbreak associated with a new coronavirus of probable bat origin
Peng Zhou,Xing-Lou Yang,Xian Guang Wang,Ben Hu,Lei Zhang,Wei Zhang,Hao Rui Si,Yan Zhu,Bei Li,Chao Lin Huang,Hui-Dong Chen,Jing Chen,Yun Luo,Hua Guo,Ren Di Jiang,Meiqin Liu,Ying Chen,Xu Rui Shen,Xi Wang,Xiao Shuang Zheng,Kai Zhao,Quanjiao Chen,Fei Deng,Lin Lin Liu,Bing Yan,Fa Xian Zhan,Yan-Yi Wang,Gengfu Xiao,Zhengli Shi +28 more
TL;DR: Identification and characterization of a new coronavirus (2019-nCoV), which caused an epidemic of acute respiratory syndrome in humans in Wuhan, China, and it is shown that this virus belongs to the species of SARSr-CoV, indicates that the virus is related to a bat coronav virus.
Journal ArticleDOI
A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster.
Jasper Fuk-Woo Chan,Shuofeng Yuan,Kin-Hang Kok,Kelvin K. W. To,Kelvin K. W. To,Hin Chu,Jin Yang,Fanfan Xing,Jieling Liu,Cyril C. Y. Yip,Rosana W.S. Poon,Hoi Wah Tsoi,Simon Kam Fai Lo,Kwok-Hung Chan,Vincent Kwok-Man Poon,Wan Mui Chan,Jonathan Daniel Ip,Jian Piao Cai,Vincent C.C. Cheng,Honglin Chen,Honglin Chen,Christopher K.M. Hui,Kwok-Yung Yuen +22 more
TL;DR: The findings are consistent with person-to-person transmission of this novel coronavirus in hospital and family settings, and the reports of infected travellers in other geographical regions.
Related Papers (5)
Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China
Chaolin Huang,Yeming Wang,Xingwang Li,Lili Ren,Jianping Zhao,Yi Hu,Li Zhang,Guohui Fan,Jiuyang Xu,Xiaoying Gu,Zhenshun Cheng,Ting Yu,Jia'an Xia,Yuan Wei,Wenjuan Wu,Xuelei Xie,Wen Yin,Li Hui,Min Liu,Yan Xiao,Hong Gao,Li Guo,Jungang Xie,Guang-Fa Wang,Rongmeng Jiang,Zhancheng Gao,Qi Jin,Jianwei Wang,Bin Cao +28 more
A pneumonia outbreak associated with a new coronavirus of probable bat origin
Peng Zhou,Xing-Lou Yang,Xian Guang Wang,Ben Hu,Lei Zhang,Wei Zhang,Hao Rui Si,Yan Zhu,Bei Li,Chao Lin Huang,Hui-Dong Chen,Jing Chen,Yun Luo,Hua Guo,Ren Di Jiang,Meiqin Liu,Ying Chen,Xu Rui Shen,Xi Wang,Xiao Shuang Zheng,Kai Zhao,Quanjiao Chen,Fei Deng,Lin Lin Liu,Bing Yan,Fa Xian Zhan,Yan-Yi Wang,Gengfu Xiao,Zhengli Shi +28 more
Clinical Characteristics of Coronavirus Disease 2019 in China.
Wei-jie Guan,Zhengyi Ni,Yu Hu,Wenhua Liang,Chun-Quan Ou,Jianxing He,Lei Liu,Hong Shan,Chunliang Lei,David S.C. Hui,Bin Du,Lanjuan Li,Guang Zeng,Kowk-Yung Yuen,Ruchong Chen,Chun-Li Tang,Tao Wang,Ping-Yan Chen,Jie Xiang,Shiyue Li,Jinlin Wang,Zi-jing Liang,Yi-xiang Peng,Li Wei,Yong Liu,Ya-hua Hu,Peng Peng,Jian-ming Wang,Ji-yang Liu,Zhong Chen,Gang Li,Zhi-jian Zheng,Shao-qin Qiu,Jie Luo,Chang-jiang Ye,Shao-yong Zhu,Nanshan Zhong +36 more