SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor
Markus Hoffmann,Hannah Kleine-Weber,Simon Schroeder,Nadine Krüger,Tanja Herrler,Sandra Erichsen,Tobias S. Schiergens,Georg Herrler,Nai Huei Wu,Andreas Nitsche,Marcel A. Müller,Christian Drosten,Christian Drosten,Stefan Pöhlmann +13 more
TLDR
It is demonstrated that SARS-CoV-2 uses the SARS -CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming, and it is shown that the sera from convalescent SARS patients cross-neutralized Sars-2-S-driven entry.About:
This article is published in Cell.The article was published on 2020-04-16 and is currently open access. It has received 15362 citations till now. The article focuses on the topics: Proteases.read more
Citations
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Distinct conformational states of SARS-CoV-2 spike protein
Yongfei Cai,Yongfei Cai,Jun Zhang,Jun Zhang,Tianshu Xiao,Tianshu Xiao,Hanqin Peng,Sarah M. Sterling,Richard M. Walsh,Shaun Rawson,Sophia Rits-Volloch,Bing Chen,Bing Chen +12 more
TL;DR: Two cryo–electron microscopy structures derived from a preparation of the full-length S protein, representing its prefusion and postfusion conformations, are reported, advancing the understanding of SARS-CoV-2 entry and may guide the development of vaccines and therapeutics.
Journal ArticleDOI
Infectious Diseases Society of America Guidelines on the Treatment and Management of Patients with COVID-19.
Adarsh Bhimraj,Rebecca L. Morgan,Amy Hirsch Shumaker,Valery Lavergne,Lindsey R. Baden,Vincent C.C. Cheng,Kathryn M. Edwards,Rajesh T. Gandhi,William J. Muller,John C. O’Horo,Shmuel Shoham,M. Hassan Murad,Reem A. Mustafa,Shahnaz Sultan,Yngve Falck-Ytter +14 more
TL;DR: It is recommended that patients be recruited into ongoing trials, which would provide much needed evidence on the efficacy and safety of various therapies for COVID-19, given that the authors could not make a determination whether the benefits outweigh harms for most treatments.
Journal ArticleDOI
Olfactory transmucosal SARS-CoV-2 invasion as a port of central nervous system entry in individuals with COVID-19.
Jenny Meinhardt,Josefine Radke,Carsten Dittmayer,Jonas Franz,Jonas Franz,Carolina Thomas,Carolina Thomas,Ronja Mothes,Michael Laue,Julia Schneider,Sebastian Brünink,Selina Greuel,Malte Lehmann,Olga Hassan,Tom Aschman,Elisa Schumann,Robert Lorenz Chua,Christian Conrad,Roland Eils,Roland Eils,Werner Stenzel,Marc Windgassen,Larissa Rößler,Hans-Hilmar Goebel,Hans R. Gelderblom,Hubert Martin,Andreas Nitsche,Walter J. Schulz-Schaeffer,Samy Hakroush,Martin Sebastian Winkler,Björn Tampe,Franziska Scheibe,Peter Körtvelyessy,Peter Körtvelyessy,Dirk Reinhold,Britta Siegmund,Anja A. Kühl,Sefer Elezkurtaj,David Horst,Lars Oesterhelweg,Michael Tsokos,Barbara Ingold-Heppner,Christine Stadelmann,Christian Drosten,Victor M. Corman,Helena Radbruch,Frank L. Heppner +46 more
TL;DR: The morphological changes associated with infection such as thromboembolic ischemic infarction of the CNS and evidence of SARS-CoV-2 neurotropism are described and presented.
Journal ArticleDOI
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection in Children and Adolescents: A Systematic Review.
Riccardo Castagnoli,Martina Votto,Amelia Licari,Ilaria Brambilla,Raffaele Bruno,Stefano Perlini,Francesca Rovida,Fausto Baldanti,Gian Luigi Marseglia +8 more
TL;DR: This is the first systematic review that assesses and summarizes clinical features and management of children with SARS-CoV-2 infection and concludes that Bronchial thickening and ground-glass opacities were the main radiologic features in asymptomatic patients.
Journal ArticleDOI
Neuropilin-1 is a host factor for SARS-CoV-2 infection.
James L. Daly,Boris Simonetti,Katja Klein,Kai-En Chen,Maia Kavanagh Williamson,Carlos Antón-Plágaro,Deborah K. Shoemark,Lorena Simón-Gracia,Michael Bauer,Reka Hollandi,Urs F. Greber,Peter Horvath,Peter Horvath,Richard B. Sessions,Ari Helenius,Julian A. Hiscox,Julian A. Hiscox,Tambet Teesalu,David A. Matthews,Andrew D. Davidson,Brett M. Collins,Peter J. Cullen,Yohei Yamauchi,Yohei Yamauchi +23 more
TL;DR: It is shown that neuropilin-1 (NRP1), which is known to bind furin-cleaved substrates, potentiates SARS-CoV-2 infectivity and serves as a host factor for Sars-Cov-2 infection and may potentially provide a therapeutic target for COVID-19.
References
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A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster.
Jasper Fuk-Woo Chan,Shuofeng Yuan,Kin-Hang Kok,Kelvin K. W. To,Kelvin K. W. To,Hin Chu,Jin Yang,Fanfan Xing,Jieling Liu,Cyril C. Y. Yip,Rosana W.S. Poon,Hoi Wah Tsoi,Simon Kam Fai Lo,Kwok-Hung Chan,Vincent Kwok-Man Poon,Wan Mui Chan,Jonathan Daniel Ip,Jian Piao Cai,Vincent C.C. Cheng,Honglin Chen,Honglin Chen,Christopher K.M. Hui,Kwok-Yung Yuen +22 more
TL;DR: The findings are consistent with person-to-person transmission of this novel coronavirus in hospital and family settings, and the reports of infected travellers in other geographical regions.
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