SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor
Markus Hoffmann,Hannah Kleine-Weber,Simon Schroeder,Nadine Krüger,Tanja Herrler,Sandra Erichsen,Tobias S. Schiergens,Georg Herrler,Nai Huei Wu,Andreas Nitsche,Marcel A. Müller,Christian Drosten,Christian Drosten,Stefan Pöhlmann +13 more
TLDR
It is demonstrated that SARS-CoV-2 uses the SARS -CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming, and it is shown that the sera from convalescent SARS patients cross-neutralized Sars-2-S-driven entry.About:
This article is published in Cell.The article was published on 2020-04-16 and is currently open access. It has received 15362 citations till now. The article focuses on the topics: Proteases.read more
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Virtual Screening of Natural Products against Type II Transmembrane Serine Protease (TMPRSS2), the Priming Agent of Coronavirus 2 (SARS-CoV-2).
Noor Naemah Abdul Rahman,Zarrin Basharat,Muhammad Yousuf,Giuseppe Castaldo,Luca Rastrelli,Luca Rastrelli,Haroon Khan +6 more
TL;DR: In vitro and in vivo validation is needed to study and develop an anti-COVID-19 drug based on the structures of the most promising compounds identified in this study.
Journal ArticleDOI
SARS-CoV-2 variants with mutations at the S1/S2 cleavage site are generated in vitro during propagation in TMPRSS2-deficient cells.
Michihito Sasaki,Kentaro Uemura,Akihiko Sato,Shinsuke Toba,Takao Sanaki,Katsumi Maenaka,William W. Hall,William W. Hall,William W. Hall,Yasuko Orba,Hirofumi Sawa,Hirofumi Sawa +11 more
TL;DR: In this paper, the authors examined the properties of SARS-CoV-2 variants with mutations at the S protein cleavage site that undergo inefficient proteolytic cleavage.
Journal ArticleDOI
Comparison of Transgenic and Adenovirus hACE2 Mouse Models for SARS-CoV-2 Infection
Raveen Rathnasinghe,Shirin Strohmeier,Fatima Amanat,Virginia L. Gillespie,Florian Krammer,Adolfo García-Sastre,Lynda Coughlan,Lynda Coughlan,Michael Schotsaert,Melissa B. Uccellini +9 more
TL;DR: The K18-hACE2 model provides a stringent model for testing vaccines and antivirals, whereas the adenovirus delivery system has the flexibility to be used across multiple genetic backgrounds and modified mouse strains.
Posted ContentDOI
Human ACE2 receptor polymorphisms predict SARS-CoV-2 susceptibility
Eric Stawiski,Devan Diwanji,Kushal Suryamohan,Ravi Gupta,Frederic A. Fellouse,Fah Sathirapongsasuti,Jiang Liu,Ying-Ping Jiang,Aakrosh Ratan,Monika Mis,Devi Santhosh,Sneha Somasekar,Sangeetha Mohan,Sameer Phalke,Boney Kuriakose,Aju Antony,Jagath Reddy Junutula,Stephan C. Schuster,Natalia Jura,Somasekar Seshagiri +19 more
TL;DR: ACE2 variants are rare, consistent with the lack of selection pressure given the recent history of SARS-CoV epidemics, however, are likely to play an important role in altering susceptibility to CoVs.
Journal ArticleDOI
Understanding SARS-CoV-2 endocytosis for COVID-19 drug repurposing.
Oleg O. Glebov,Oleg O. Glebov +1 more
TL;DR: Taking advantage of the well‐established methodology of membrane trafficking studies, this research direction allows for the rapid characterisation of the key cell biological mechanism(s) responsible for SARS‐CoV‐2 infection.
References
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TL;DR: The findings are consistent with person-to-person transmission of this novel coronavirus in hospital and family settings, and the reports of infected travellers in other geographical regions.
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