Small extracellular vesicles-based cell-free strategies for therapy
TLDR
This work summarizes applications of sEVs‐based therapies in different diseases and current knowledge about the strategies in bioengineered s EVs, as well as the challenges for their use in clinical settings.Abstract:
Small extracellular vesicles (sEVs) are extracellular nanovesicles that contain bioactive proteins, lipids, RNA, and DNA. A variety of biological process is regulated with sEVs. sEVs are an intercellular messenger regulating recipient cell function and play a role in disease initiation and progression. sEVs derived from certain cells, such as mesenchymal stem cells and immune cells, have the potential for clinical therapy as they possess the characteristics of their parental cells. With better understanding of sEVs biogenesis, their transportation properties, extended circulatory capability, and exceptional biocompatibility, sEVs emerge as a potential therapeutic tool in the clinic. Here, we summarize applications of sEVs-based therapies in different diseases and current knowledge about the strategies in bioengineered sEVs, as well as the challenges for their use in clinical settings.read more
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Diagnostic and Therapeutic Potential of Circulating-Free DNA and Cell-Free RNA in Cancer Management
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OUP accepted manuscript
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Strategies for improving adipose-derived stem cells for tissue regeneration
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Enterovirus 71 non-structural protein 3A hijacks vacuolar protein sorting 25 to boost exosome biogenesis to facilitate viral replication
Zhihui Ruan,Yicong Liang,Zicong Chen,Jialing Yin,Chengcheng Li,Pan Pan,Qiwei Zhang,Jianguo Wu,Zhen Luo +8 more
TL;DR: A novel mechanism underlying EV71-mediated exosomes in the regulation of viral replication is uncovered, which provides potential anti-viral strategies against the EV71 infection and transmission in HFMD.
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Neural stem/progenitor cell‐derived extracellular vesicles: A novel therapy for neurological diseases and beyond
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TL;DR: The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities, and a checklist is provided with summaries of key points.
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Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
TL;DR: This unit describes different approaches for exosome purification from various sources, and discusses methods to evaluate the purity and homogeneity of the purified exosomes preparations.