A library of induced pluripotent stem cells from clinically well-characterized, diverse healthy human individuals
Christoph Schaniel,Priyanka Dhanan,Bin Hu,Yuguang Xiong,Teeya Raghunandan,David M. Gonzalez,Rafael Dariolli,Sunita L. D’Souza,Arjun Singh Yadaw,Jens Hansen,Gomathi Jayaraman,Bino Mathew,Moara Machado,Seth I. Berger,Joseph Tripodig,Vesna Najfeld,Jalaj Garg,Marc A. Miller,Colleen S Surlyn,Katherine C. Michelis,Neelima C. Tangirala,Himali Weerahandi,David C. Thomas,Kristin G. Beaumont,Robert Sebra,Milind Mahajan,Eric E. Schadt,Dusica Vidovic,Stephan C. Schürer,Joseph Goldfarb,Evren U. Azeloglu,Marc R. Birtwistle,Eric A. Sobie,Jason C. Kovacic,Nicole Dubois,Nicole Dubois,Ravi Iyengar +36 more
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A gender-balanced, racially/ethnically diverse library of human induced pluripotent stem cells (hiPSC) lines from 40 clinically healthy human individuals who range in age from 22 to 61 years was reported in this article.Abstract:
Summary A library of well-characterized human induced pluripotent stem cell (hiPSC) lines from clinically healthy human subjects could serve as a useful resource of normal controls for in vitro human development, disease modeling, genotype-phenotype association studies, and drug response evaluation. We report generation and extensive characterization of a gender-balanced, racially/ethnically diverse library of hiPSC lines from 40 clinically healthy human individuals who range in age from 22 to 61 years. The hiPSCs match the karyotype and short tandem repeat identities of their parental fibroblasts, and have a transcription profile characteristic of pluripotent stem cells. We provide whole-genome sequencing data for one hiPSC clone from each individual, genomic ancestry determination, and analysis of mendelian disease genes and risks. We document similar transcriptomic profiles, single-cell RNA-sequencing-derived cell clusters, and physiology of cardiomyocytes differentiated from multiple independent hiPSC lines. This extensive characterization makes this hiPSC library a valuable resource for many studies on human biology.read more
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PPARdelta activation induces metabolic and contractile maturation of human pluripotent stem-cell-derived cardiomyocytes.
Nadeera Wickramasinghe,David H. Sachs,Bhavana Shewale,David M. Gonzalez,Priyanka Dhanan-Krishnan,Denis Torre,Elizabeth LaMarca,Serena Raimo,Rafael Dariolli,Madhavika N. Serasinghe,Joshua Mayourian,Robert Sebra,Kristin G. Beaumont,Srinivas Iyengar,Deborah L. French,Arne Hansen,Thomas Eschenhagen,Jerry E. Chipuk,Eric A. Sobie,Adam Jacobs,Schahram Akbarian,Harry Ischiropoulos,Avi Ma'ayan,Sander M. Houten,Kevin Costa,Nicole Dubois +25 more
TL;DR: In this paper , peroxisome-proliferator-associated receptor (PPAR) signaling regulates glycolysis and fatty acid oxidation (FAO) in an isoform-specific manner.
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Functional Effects of Cardiomyocyte Injury in COVID-19
TL;DR: In this article , the authors integrated cell biological and physiological analyses of human cardiomyocytes differentiated from human induced pluripotent stem cells (hiPSCs) infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the presence of interleukins (ILs) with clinical findings related to laboratory values in COVID-19 patients.
Journal ArticleDOI
Fibroblast growth factor homologous factors serve as a molecular rheostat in tuning arrhythmogenic cardiac late sodium current
Nourdine Chakouri,Sharen Rivas,Daniel Roybal,Lin Yang,Johanna Diaz,Allen L. Hsu,Ryan Mahling,Biyi Chen,Josiah O. Owoyemi,Deborah DiSilvestre,Dario Sirabella,Barbara Corneo,Gordon F. Tomaselli,Ivy E. Dick,Steven O. Marx,Manu Ben-Johny +15 more
TL;DR: In this article , the authors demonstrate that the intracellular fibroblast growth factor homologous factors (FHF1−4) tune pathogenic late sodium current (INa,L) in an isoform-specific manner.
Journal ArticleDOI
Action potential variability in human pluripotent stem cell-derived cardiomyocytes obtained from healthy donors
Anna-Bella Carvalho,Keyla Cristiny da Silva Coutinho,Raiana A. Q. Barbosa,Dilza Balteiro Pereira de Campos,Isabela de Carvalho Leitão,Rafael Santos Pinto,Danúbia Silva dos Santos,Bruna Farjun,Dayanada Silva de Araújo,Fernanda Mesquita,Gustavo Monnerat-Cahli,Emiliano Medei,Tais Hanae Kasai-Brunswick,A C de Carvalho +13 more
TL;DR: In this article , the authors report the action potential variability of 780 cardiomyocytes derived from pluripotent stem cells obtained from six healthy donors and conclude that even using the same cell line and differentiation protocol, the differentiation batch still affects the results.
Journal ArticleDOI
Harshening stem cell research and precision medicine: The states of human pluripotent cells stem cell repository diversity, and racial and sex differences in transcriptomes
TL;DR: This paper analyzed the diversity of human induced pluripotent stem cells with respect to ethnicity, sex, and disease types, and revealed a lack of diversity despite the large sample size of human ILP cells and showed that the White group made up the majority of the banked ILP stem cells.
References
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Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA
Luigi Warren,Philip D. Manos,Philip D. Manos,Tim Ahfeldt,Tim Ahfeldt,Yuin-Han Loh,Hu Li,Hu Li,Frank H. Lau,Wataru Ebina,Pankaj Mandal,Zachary D. Smith,Alexander Meissner,Alexander Meissner,George Q. Daley,Andrew S. Brack,James J. Collins,James J. Collins,James J. Collins,Chad A. Cowan,Thorsten M. Schlaeger,Thorsten M. Schlaeger,Derrick J. Rossi +22 more
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ClinVar: public archive of relationships among sequence variation and human phenotype
Melissa J. Landrum,Jennifer M. Lee,George R. Riley,Wonhee Jang,Wendy S. Rubinstein,Deanna M. Church,Donna Maglott +6 more
TL;DR: To facilitate evaluation of the medical importance of each variant, ClinVar aggregates submissions with the same variation/phenotype combination, adds value from other NCBI databases, assigns a distinct accession of the format RCV000000000.0 and reports if there are conflicting clinical interpretations.
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