RNA-Seq: a revolutionary tool for transcriptomics
TLDR
The RNA-Seq approach to transcriptome profiling that uses deep-sequencing technologies provides a far more precise measurement of levels of transcripts and their isoforms than other methods.Abstract:
RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.read more
Citations
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New Technologies for Studying Biofilms.
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The Pseudomonas aeruginosa Transcriptome in Planktonic Cultures and Static Biofilms Using RNA Sequencing
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Deep proteomics of the Xenopus laevis egg using an mRNA-derived reference database.
Martin Wühr,Robert Freeman,Marc Presler,Marko E. Horb,Leonid Peshkin,Steven P. Gygi,Marc W. Kirschner +6 more
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Simultaneous RNA-seq analysis of a mixed transcriptome of rice and blast fungus interaction.
Yoshihiro Kawahara,Youko Oono,Hiroyuki Kanamori,Takashi Matsumoto,Takeshi Itoh,Eiichi Minami +5 more
TL;DR: The mixed transcriptome analysis of rice and blast fungus in infected leaves at 24 hours post-inoculation is demonstrated, showing that the gene expression of both the host plant and pathogen simultaneously in the same infected leaf blades in natural infection conditions without any artificial treatments is detected.
RNA-sequencing From Single Nuclei
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