RNA-Seq: a revolutionary tool for transcriptomics
TLDR
The RNA-Seq approach to transcriptome profiling that uses deep-sequencing technologies provides a far more precise measurement of levels of transcripts and their isoforms than other methods.Abstract:
RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.read more
Citations
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Revisiting Global Gene Expression Analysis
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TL;DR: In this article, the authors discuss common assumptions currently embedded in experimental and analytical practices that can lead to misinterpretation of global gene expression data and describe solutions that should minimize erroneous interpretation of gene expression from multiple analysis platforms.
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Ribosome Profiling Reveals Pervasive Translation Outside of Annotated Protein-Coding Genes
Nicholas T. Ingolia,Gloria A. Brar,Noam Stern-Ginossar,Michael S. Harris,Michael S. Harris,Gaëlle J.S. Talhouarne,Gaëlle J.S. Talhouarne,Sarah E. Jackson,Mark R. Wills,Jonathan S. Weissman +9 more
TL;DR: Hallmarks of translation in ribosome footprints are shown: copurification with the large ribosomal subunit, response to drugs targeting elongation, trinucleotide periodicity, and initiation at early AUGs.
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