RNA-Seq: a revolutionary tool for transcriptomics
TLDR
The RNA-Seq approach to transcriptome profiling that uses deep-sequencing technologies provides a far more precise measurement of levels of transcripts and their isoforms than other methods.Abstract:
RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.read more
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AMPKα modulation in cancer progression: Multilayer integrative analysis of the whole transcriptome in Asian gastric cancer
Yon Hui Kim,Han Liang,Xiuping Liu,Ju Seog Lee,Jae Yong Cho,Jae Ho Cheong,Hoguen Kim,Min Li,Thomas J. Downey,Matthew D. Dyer,Yongming Sun,Jingtao Sun,Ellen M. Beasley,Hyun Cheol Chung,Sung Hoon Noh,John N. Weinstein,Chang Gong Liu,Garth Powis +17 more
TL;DR: In this article, an RNA-sequencing approach was applied to gastric tumor and non-cancerous specimens, generating 680 million informative short reads to quantitatively characterize the entire transcriptome of gastric cancer (including mRNAs and microRNAs).
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TL;DR: The results suggest that transcriptome modifications can be selected rapidly by insecticides and affect multiple biological functions, and polymorphism variations of detoxification enzymes may play an important role in the adaptive response of mosquitoes to insecticides.
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