RNA-Seq: a revolutionary tool for transcriptomics
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TLDR
The RNA-Seq approach to transcriptome profiling that uses deep-sequencing technologies provides a far more precise measurement of levels of transcripts and their isoforms than other methods.Abstract:
RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.read more
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Massive parallel sequencing of mRNA in identification of unannotated salinity stress-inducible transcripts in rice (Oryza sativa L.)
Hiroshi Mizuno,Yoshihiro Kawahara,Hiroaki Sakai,Hiroyuki Kanamori,Hironobu Wakimoto,Harumi Yamagata,Youko Oono,Jianzhong Wu,Hiroshi Ikawa,Takeshi Itoh,Takashi Matsumoto +10 more
TL;DR: In this article, a series of programs (Bowtie, TopHat, and Cufflinks) comprehensively predicted 51,301 (shoot) and 54,491 (root) transcripts from the Rice Annotation Project database.
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Evolving Strategies for Target Selection for Antibody-Drug Conjugates
TL;DR: The tumor and normal tissue expression profiles of the targets of ADCs currently in clinical development are surveyed, demonstrating a surprisingly broad range of expression profiles and the inability to formalize any optimal parameters for an ADC target.
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Transcriptomic Analysis of the Salivary Glands of an Invasive Whitefly
TL;DR: The combined method of cDNA amplification, Illumina sequencing and de novo assembly is suitable for transcriptomic analysis of tiny organs in insects and will be the basis for future studies on whitefly-plant interactions and virus transmission.
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Fighting a Losing Battle: Vigorous Immune Response Countered by Pathogen Suppression of Host Defenses in the Chytridiomycosis-Susceptible Frog Atelopus zeteki
Amy Ellison,Anna E. Savage,Grace V. DiRenzo,Penny F. Langhammer,Karen R. Lips,Kelly R. Zamudio +5 more
TL;DR: The authors found significant changes in expression of numerous genes involved in innate and inflammatory responses in infected frogs despite high susceptibility to chytridiomycosis, including increased expression of immunoglobulins and major histocompatibility complex (HCC) genes.
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Development of the catfish 250K SNP array for genome-wide association studies
Shikai Liu,Luyang Sun,Yun Li,Fanyue Sun,Yanliang Jiang,Yu Zhang,Jiaren Zhang,Jianbin Feng,Ludmilla Kaltenboeck,Huseyin Kucuktas,Zhanjiang Liu +10 more
TL;DR: This work developed a catfish 250K SNP array using Affymetrix Axiom genotyping technology, which is the first high-density SNP array for catfish and should be a valuable resource for genome-wide association studies (GWAS), fine QTL mapping, high- density linkage map construction, haplotype analysis, and whole genome-based selection.
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