RNA-Seq: a revolutionary tool for transcriptomics
TLDR
The RNA-Seq approach to transcriptome profiling that uses deep-sequencing technologies provides a far more precise measurement of levels of transcripts and their isoforms than other methods.Abstract:
RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.read more
Citations
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Methods for biological data integration: perspectives and challenges
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Comparative transcriptomics of three Poaceae species reveals patterns of gene expression evolution.
Rebecca M. Davidson,Malali Gowda,Gaurav D. Moghe,Haining Lin,Brieanne Vaillancourt,Shin-Han Shiu,Ning Jiang,C. Robin Buell +7 more
TL;DR: In this paper, the authors employed a sequence-based approach to compare analogous transcriptomes in species representing three Poaceae subgroups including the Pooideae (Brachypodium distachyon), the Panicoidesae (sorghum), and the Ehrhartoidea (rice), and showed that only a fraction of orthologous genes exhibit conserved expression patterns.
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