RNA-Seq: a revolutionary tool for transcriptomics
TLDR
The RNA-Seq approach to transcriptome profiling that uses deep-sequencing technologies provides a far more precise measurement of levels of transcripts and their isoforms than other methods.Abstract:
RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.read more
Citations
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Global Analysis of the Transcriptional Response of Whitefly to Tomato Yellow Leaf Curl China Virus Reveals the Relationship of Coevolved Adaptations
Jun-Bo Luan,Junmin Li,Nélia Varela,Yong-Liang Wang,Fangfang Li,Yan-Yuan Bao,Chuan-Xi Zhang,Shu-Sheng Liu,Xiao-Wei Wang +8 more
TL;DR: The relationship of coevolved adaptations between begomoviruses and whiteflies is revealed and will provide a road map for future investigations into the complex interactions between plant viruses and their insect vectors.
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A Genome-Wide Regulatory Framework Identifies Maize Pericarp Color1 Controlled Genes
Kengo Morohashi,María Isabel Casas,Lorena Falcone Ferreyra,María Katherine Mejía-Guerra,Lucille Pourcel,Alper Yilmaz,Alper Yilmaz,Antje Feller,Bruna Carvalho,Julia Emiliani,Eduardo Rodriguez,Silvina C. Pellegrinet,Michael D. McMullen,Paula Casati,Erich Grotewold +14 more
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The emerging role of circular RNAs in transcriptome regulation
TL;DR: A comprehensive overview on the biology of circRNAs, including the expression patterns and function is provided, and current methodologies for the discovery and validation of circular transcripts are discussed.
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RNA-Seq Analysis of Human Trigeminal and Dorsal Root Ganglia with a Focus on Chemoreceptors
Caroline Flegel,Nicole Schöbel,Janine Altmüller,Christian Becker,Andrea Tannapfel,Hanns Hatt,Günter Gisselmann +6 more
TL;DR: The next-generation sequencing technique (RNA-Seq) is employed to conduct the first expression analysis of human trigeminal ganglia and dorsal root ganglia, and gives an overview of the major classes of chemoreceptors expressed in the human TG and DRG and provides the basis for a broader understanding of the reception of chemical cues.
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Improving metabolic flux predictions using absolute gene expression data
Dave Lee,Kieran Smallbone,Warwick B. Dunn,Ettore Murabito,Catherine L. Winder,Douglas B. Kell,Pedro Mendes,Pedro Mendes,Neil Swainston +8 more
TL;DR: Using quantitative transcriptomics data acquired from Saccharomyces cerevisiae cultures under two growth conditions, the method outperforms traditional approaches for predicting experimentally measured exometabolic flux that are reliant upon maximisation of the rate of biomass production.
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