Journal ArticleDOI
The emerging field of lipidomics
TLDR
Novel analytical approaches — in particular, liquid chromatography and mass spectrometry — for systems-level analysis of lipids and their interacting partners (lipidomics) now make this field a promising area of biomedical research, with a variety of applications in drug and biomarker development.Abstract:
The crucial role of lipids in cell, tissue and organ physiology is demonstrated by a large number of genetic studies and by many human diseases that involve the disruption of lipid metabolic enzymes and pathways. Examples of such diseases include cancer, diabetes, as well as neurodegenerative and infectious diseases. So far, the explosion of information in the fields of genomics and proteomics has not been matched by a corresponding advancement of knowledge in the field of lipids, which is largely due to the complexity of lipids and the lack of powerful tools for their analysis. Novel analytical approaches--in particular, liquid chromatography and mass spectrometry--for systems-level analysis of lipids and their interacting partners (lipidomics) now make this field a promising area of biomedical research, with a variety of applications in drug and biomarker development.read more
Citations
More filters
Journal ArticleDOI
Characterization and classification of rat neural stem cells and differentiated cells by comparative metabolic and lipidomic profiling.
TL;DR: The results of this study suggest objective and complementary criteria for the characterization and classification of NSCs and DCs for potential clinical applications.
Journal ArticleDOI
Using stable isotope tracers to monitor membrane dynamics in C. elegans.
TL;DR: Analytical methods that increase the capacity to map the dynamics of individual membrane phospholipids using different types of mass spectrometry are described, including novel stable isotope (13C and 15N) strategies to quantify the turnover of dozens of fatty acid tails and intact phospholIPids simultaneously.
Book ChapterDOI
Chapter 8 – Lipidomics
TL;DR: A typical work-flow diagram for lipidomics study involve lipid extraction from the matrix, ionization in a proper source, and subsequent mass spectrometry analysis by direct infusion or after a front-end separation step.
Journal ArticleDOI
The human extended mitochondrial metabolic network: New hubs from lipids
TL;DR: It is shown that, considering combinatorial diversity oflipids and multipurpose enzymes, an intimate connection between membrane lipids and oxidative phosphorilation appears, and this finding leads to some biomedical considerations on diseases involving mitochondrial enzymes.
Journal ArticleDOI
A new metabonomics method for simultaneous determination of EFAs and NEFAs in plasma using GC–MS and its application
TL;DR: In this article, a new metabonomics method was developed for simultaneous qualitative and quantitative analysis on esterified and nonesterified fatty acids (EFAs and NEFAs) in plasma.
References
More filters
Journal ArticleDOI
A rapid method of total lipid extraction and purification.
E. G. Bligh,W. J. Dyer +1 more
TL;DR: The lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials that has been applied to fish muscle and may easily be adapted to use with other tissues.
Journal ArticleDOI
Functional rafts in cell membranes
Kai Simons,Elina Ikonen +1 more
TL;DR: A new aspect of cell membrane structure is presented, based on the dynamic clustering of sphingolipids and cholesterol to form rafts that move within the fluid bilayer that function as platforms for the attachment of proteins when membranes are moved around inside the cell and during signal transduction.
Journal ArticleDOI
Toll-like receptor signalling
Shizuo Akira,Kiyoshi Takeda +1 more
TL;DR: Rapid progress that has recently improved the understanding of the molecular mechanisms that mediate TLR signalling is reviewed.
Journal ArticleDOI
Defective LPS Signaling in C3H/HeJ and C57BL/10ScCr Mice: Mutations in Tlr4 Gene
Alexander Poltorak,Xiaolong He,Irina Smirnova,Mu Ya Liu,Christophe Van Huffel,Xin Du,Dale Birdwell,E. Alejos,M. Silva,Chris Galanos,Marina Freudenberg,Paola Ricciardi-Castagnoli,Betsy Layton,Bruce Beutler +13 more
TL;DR: The mammalian Tlr4 protein has been adapted primarily to subserve the recognition of LPS and presumably transduces the LPS signal across the plasma membrane.
Related Papers (5)
Shotgun lipidomics: Electrospray ionization mass spectrometric analysis and quantitation of cellular lipidomes directly from crude extracts of biological samples
Xianlin Han,Richard W. Gross +1 more
A comprehensive classification system for lipids
Eoin Fahy,Shankar Subramaniam,H. Alex Brown,Christopher K. Glass,Alfred H. Merrill,Robert C. Murphy,Christian R. H. Raetz,David W. Russell,Yousuke Seyama,Walter A. Shaw,Takao Shimizu,Friedrich Spener,Gerrit van Meer,Michael S. VanNieuwenhze,Stephen H. White,Joseph L. Witztum,Edward A. Dennis +16 more