Journal ArticleDOI
The emerging field of lipidomics
TLDR
Novel analytical approaches — in particular, liquid chromatography and mass spectrometry — for systems-level analysis of lipids and their interacting partners (lipidomics) now make this field a promising area of biomedical research, with a variety of applications in drug and biomarker development.Abstract:
The crucial role of lipids in cell, tissue and organ physiology is demonstrated by a large number of genetic studies and by many human diseases that involve the disruption of lipid metabolic enzymes and pathways. Examples of such diseases include cancer, diabetes, as well as neurodegenerative and infectious diseases. So far, the explosion of information in the fields of genomics and proteomics has not been matched by a corresponding advancement of knowledge in the field of lipids, which is largely due to the complexity of lipids and the lack of powerful tools for their analysis. Novel analytical approaches--in particular, liquid chromatography and mass spectrometry--for systems-level analysis of lipids and their interacting partners (lipidomics) now make this field a promising area of biomedical research, with a variety of applications in drug and biomarker development.read more
Citations
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Journal ArticleDOI
In vivo metabolic fingerprinting of neutral lipids with hyperspectral stimulated Raman scattering microscopy
Dan Fu,Yong Yu,Andrew Folick,Erin Currie,Robert V. Farese,Tsung-Huang Tsai,Xiaoliang Sunney Xie,Meng C. Wang +7 more
TL;DR: It is found that unsaturated fatty acid has preferential uptake into lipid storage while saturated fatty acid exhibits toxicity in hepatic cells, unlike previously hypothesized.
Journal ArticleDOI
Metabolomics, a novel tool for studies of nutrition, metabolism and lipid dysfunction
TL;DR: Innovative experimental designs combined with novel computational tools for handling metabolomics data offer new opportunities for early disease detection as well as for characterization of dietary and therapeutic interventions in the context of human physiology.
Journal ArticleDOI
MALDI-TOF MS in lipidomics.
TL;DR: This review starts with a short overview on traditional methods of lipid analysis with the focus on mass spectrometric methods and compares MALDI-TOF MS with other important ionization techniques and focuses on the advantages and drawbacks of MalDI-toF MS in lipidomics.
Journal ArticleDOI
Meibum Lipid Composition in Asians with Dry Eye Disease
Sin Man Lam,Louis Tong,Siew Sian Yong,Bowen Li,Shyam S. Chaurasia,Guanghou Shui,Markus R. Wenk +6 more
TL;DR: A comprehensive lipidome of meibum from individuals of an Asian ethnicity is presented, for the first time, which can potentially offer new insights into the higher prevalence of dysfunctional tear syndrome observed amongst Asian populations.
Journal ArticleDOI
Lipids of human meibum: mass-spectrometric analysis and structural elucidation
TL;DR: Observations suggest that MGS are a major source of nonpolar lipids of the THE AUTHORS and Chl-E families for the tear film lipid layer, but not of its previously reported (phospho)lipid, Cer, and fatty acid amide components.
References
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