Journal ArticleDOI
The emerging field of lipidomics
TLDR
Novel analytical approaches — in particular, liquid chromatography and mass spectrometry — for systems-level analysis of lipids and their interacting partners (lipidomics) now make this field a promising area of biomedical research, with a variety of applications in drug and biomarker development.Abstract:
The crucial role of lipids in cell, tissue and organ physiology is demonstrated by a large number of genetic studies and by many human diseases that involve the disruption of lipid metabolic enzymes and pathways. Examples of such diseases include cancer, diabetes, as well as neurodegenerative and infectious diseases. So far, the explosion of information in the fields of genomics and proteomics has not been matched by a corresponding advancement of knowledge in the field of lipids, which is largely due to the complexity of lipids and the lack of powerful tools for their analysis. Novel analytical approaches--in particular, liquid chromatography and mass spectrometry--for systems-level analysis of lipids and their interacting partners (lipidomics) now make this field a promising area of biomedical research, with a variety of applications in drug and biomarker development.read more
Citations
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Journal ArticleDOI
Profiling of lipid species by normal-phase liquid chromatography, nanoelectrospray ionization, and ion trap-orbitrap mass spectrometry
Elena Sokol,Reinaldo Almeida,Hans Kristian Hannibal-Bach,Dorota Ewa Kotowska,Johannes Vogt,Jan Baumgart,Karsten Kristiansen,Robert Nitsch,Jens Knudsen,Christer S. Ejsing +9 more
TL;DR: The reproducible, sensitive, and integrated lipidomics workflow based on normal-phase liquid chromatography-Fourier transform mass spectrometry and LC-ITMS(2) for profiling and structural analysis of lipid species is devised and can be extended for detailed lipid analysis of sample matrices having a wide range of lipid compositions.
Journal ArticleDOI
An editor for pathway drawing and data visualization in the Biopathways Workbench
TL;DR: Inclusion of access to multiple experimental data types and of pathway diagrams within a single interface, automatic updating through connectivity to an online database, and a focus on annotation, supports the view that the Pathway Editor represents a significant, practicable contribution to current pathway modeling tools.
Journal ArticleDOI
Lipids in Health and Disease
TL;DR: An inverse relationship between plasma lipid levels and patients was found in oral submucous fibrosis patients and the lower levels of plasma cholesterol and other lipid constituents in patients might be due to their increased utilization.
Book ChapterDOI
Use of acid sphingomyelinase for cancer therapy.
TL;DR: Emerging preclinical efficacy studies using rhASM as an adjunct in the treatment of solid tumors are summarized, and the potential for pharmacological modulation using ASM is discussed.
Journal ArticleDOI
Separation and identification of phospholipids by hydrophilic interaction liquid chromatography coupled to tandem high resolution mass spectrometry with focus on isomeric phosphatidylglycerol and bis(monoacylglycero)phosphate
TL;DR: An extended phospholipid profiling method based on hydrophilic interaction liquid chromatography coupled to high-resolution mass spectrometry (MS) and data-dependent MS/MS acquisition was developed to separate and unambiguously identify BMP species.
References
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