Journal ArticleDOI
The emerging field of lipidomics
TLDR
Novel analytical approaches — in particular, liquid chromatography and mass spectrometry — for systems-level analysis of lipids and their interacting partners (lipidomics) now make this field a promising area of biomedical research, with a variety of applications in drug and biomarker development.Abstract:
The crucial role of lipids in cell, tissue and organ physiology is demonstrated by a large number of genetic studies and by many human diseases that involve the disruption of lipid metabolic enzymes and pathways. Examples of such diseases include cancer, diabetes, as well as neurodegenerative and infectious diseases. So far, the explosion of information in the fields of genomics and proteomics has not been matched by a corresponding advancement of knowledge in the field of lipids, which is largely due to the complexity of lipids and the lack of powerful tools for their analysis. Novel analytical approaches--in particular, liquid chromatography and mass spectrometry--for systems-level analysis of lipids and their interacting partners (lipidomics) now make this field a promising area of biomedical research, with a variety of applications in drug and biomarker development.read more
Citations
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Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics
TL;DR: In this paper, the methyl-tert-butyl ether (MTBE) extraction method was used for shotgun profiling of lipid species, and the results showed that the MTBE protocol delivered similar or better recoveries of species of most major lipid classes compared with the "gold-standard" Folch or Bligh and Dyer recipes.
Journal ArticleDOI
The Human Serum Metabolome
Nikolaos Psychogios,David Hau,Jun Peng,An Chi Guo,Rupasri Mandal,Souhaila Bouatra,Igor Sinelnikov,Ramanarayan Krishnamurthy,Roman Eisner,Bijaya Gautam,Nelson Young,Jianguo Xia,Craig Knox,Edison Dong,Paul Huang,Zsuzsanna Hollander,Theresa L. Pedersen,Steven R. Smith,Fiona Bamforth,Russell Greiner,Bruce M. McManus,John W. Newman,Theodore L. Goodfriend,David S. Wishart,David S. Wishart +24 more
TL;DR: This work has combined targeted and non-targeted NMR, GC-MS and LC-MS methods with computer-aided literature mining to identify and quantify a comprehensive, if not absolutely complete, set of metabolites commonly detected and quantified (with today's technology) in the human serum metabolome.
Journal ArticleDOI
Global analysis of the yeast lipidome by quantitative shotgun mass spectrometry
Christer S. Ejsing,Julio L. Sampaio,Vineeth Surendranath,Eva Duchoslav,Kim Ekroos,Robin W. Klemm,Kai Simons,Andrej Shevchenko +7 more
TL;DR: Comparative lipidomics demonstrated that growth temperature and defects in lipid biosynthesis induce ripple effects throughout the molecular composition of the yeast lipidome, establishing shotgun lipidomics as a powerful platform for complementing biochemical studies and other systems-level approaches.
Journal ArticleDOI
ABC transporters in cancer: more than just drug efflux pumps.
TL;DR: It is proposed that delineating the precise roles of these transporters in tumorigenesis and treatment response will be important for the development of more effective targeted therapies.
Journal ArticleDOI
The model organism as a system: integrating 'omics' data sets.
TL;DR: Researchers are rising to the challenge by using omics data integration to address fundamental biological questions that would increase the understanding of systems as a whole.
References
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Journal ArticleDOI
Phosphoinositide Recognition Domains
TL;DR: Domains or modules known to bind phosphoinositides have increased dramatically in number over the past few years, and are found in proteins involved in intracellular trafficking, cellular signaling, and cytoskeletal remodeling.
Journal ArticleDOI
Essential Role for Cholesterol in Entry of Mycobacteria into Macrophages
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Journal ArticleDOI
The transcriptional responses of Mycobacterium tuberculosis to inhibitors of metabolism: novel insights into drug mechanisms of action.
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TL;DR: The transcriptional profile generated by a crude marine natural product recapitulated the mechanistic prediction from the pure active component of Mycobacterium tuberculosis and provide a rational basis for the selection of critical metabolic targets for screening for new agents with improved activity against this important human pathogen.
Journal ArticleDOI
A comprehensive classification system for lipids
Eoin Fahy,Shankar Subramaniam,H. Alex Brown,Christopher K. Glass,Alfred H. Merrill,Robert C. Murphy,Christian R. H. Raetz,David W. Russell,Yousuke Seyama,Walter A. Shaw,Takao Shimizu,Friedrich Spener,Gerrit van Meer,Michael S. VanNieuwenhze,Stephen H. White,Joseph L. Witztum,Edward A. Dennis +16 more
TL;DR: A structured vocabulary will facilitate the systematization of lipid biology and enable the cataloging of lipids and their properties in a way that is compatible with other macromolecular databases.
Journal ArticleDOI
Lipopenia and skin barrier abnormalities in DGAT2-deficient mice.
S. Stone,Heather M. Myers,Steven M. Watkins,Barbara E. Brown,Kenneth R. Feingold,Peter M. Elias,Peter M. Elias,Robert V. Farese +7 more
TL;DR: Dgat2-deficient mice are lipopenic and die soon after birth, apparently from profound reductions in substrates for energy metabolism and from impaired permeability barrier function in the skin, supporting the hypothesis that the two enzymes play fundamentally different roles in mammalian triglyceride metabolism.
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