Journal ArticleDOI
The emerging field of lipidomics
TLDR
Novel analytical approaches — in particular, liquid chromatography and mass spectrometry — for systems-level analysis of lipids and their interacting partners (lipidomics) now make this field a promising area of biomedical research, with a variety of applications in drug and biomarker development.Abstract:
The crucial role of lipids in cell, tissue and organ physiology is demonstrated by a large number of genetic studies and by many human diseases that involve the disruption of lipid metabolic enzymes and pathways. Examples of such diseases include cancer, diabetes, as well as neurodegenerative and infectious diseases. So far, the explosion of information in the fields of genomics and proteomics has not been matched by a corresponding advancement of knowledge in the field of lipids, which is largely due to the complexity of lipids and the lack of powerful tools for their analysis. Novel analytical approaches--in particular, liquid chromatography and mass spectrometry--for systems-level analysis of lipids and their interacting partners (lipidomics) now make this field a promising area of biomedical research, with a variety of applications in drug and biomarker development.read more
Citations
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Journal ArticleDOI
Comparison of lipidome profiles of Caenorhabditis elegans—results from an inter-laboratory ring trial
Britta Spanier,Anne Laurençon,Anna Weiser,Nathalie Pujol,Shizue Omi,Aiko Barsch,Ansgar Korf,Sven W. Meyer,Jonathan J. Ewbank,Francesca Paladino,Steve Garvis,Hugo Aguilaniu,Michael Witting +12 more
TL;DR: In this article, an inter-laboratory study was conducted to compare the lipid profiles of N2 wild type C. elegans and daf-2(e1370) mutants lacking a functional insulin receptor.
Journal ArticleDOI
Singapore R&D and globetrotting
TL;DR: By discussing the integrative nature of chemistry and biology, this work shall attempt to address both local and international perspectives and give an overview of the funding structure and collaborative opportunities in Singapore.
Journal ArticleDOI
A class of anti-inflammatory lipids decrease with aging in the central nervous system
Dan Tan,Srihari Konduri,Meric Erikci Ertunc,Pan Zhang,Justin Wang,Ti-Yu Chang,Antonio Pinto,Andrea Rocha,Cynthia J. Donaldson,Joan Vaughan,Raissa G. Ludwig,Elizabeth Willey,Manasi Iyer,Peter C. Gray,Pamela Maher,Nicola J. Allen,J. Bradley Zuchero,Andrew Dillin,Marcelo A. Mori,Steven G. Kohama,Dionicio Siegel,Alan Saghatelian +21 more
TL;DR: The brain lipidome of mice across their lifespan is examined using untargeted lipidomics and it is found that an SGDG dramatically suppresses LPS-induced gene expression and release of pro-inflammatory cytokines from macrophages and microglia by acting on the NF-κB pathway.
Book ChapterDOI
Supercritical Fluid Chromatography
Udi Jumhawan,Takeshi Bamba +1 more
TL;DR: In this paper, supercritical fluid chromatography (SFC) is a separation technique that characteristically a hybrid of both gas chromatography and liquid chromatography, with the favorable properties of green solvent supercritical carbon dioxide as the main mobile phase, SFC has gained much attraction for analyzing and separating a wide range of compounds.
Journal ArticleDOI
Involvement of cell shape and lipid metabolism in glioblastoma resistance to temozolomide
Munki Choo,Van-Hieu Mai,Han Sun Kim,Dong Hwa Kim,Ja Lok Ku,Sang Kook Lee,Chul-Kee Park,Yong Jin An,Sunghyouk Park +8 more
TL;DR: The transcriptomic analysis demonstrated that lipid synthesis pathways through SREBP were upregulated in U87R cells, which was confirmed at the protein level, and NMR-based lipid metabolomics revealed a significant increase in cholesterol and fatty acid synthesis as well as lower lipid unsaturation in U 87R cells.
References
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TL;DR: The mammalian Tlr4 protein has been adapted primarily to subserve the recognition of LPS and presumably transduces the LPS signal across the plasma membrane.
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