Journal ArticleDOI
The emerging field of lipidomics
TLDR
Novel analytical approaches — in particular, liquid chromatography and mass spectrometry — for systems-level analysis of lipids and their interacting partners (lipidomics) now make this field a promising area of biomedical research, with a variety of applications in drug and biomarker development.Abstract:
The crucial role of lipids in cell, tissue and organ physiology is demonstrated by a large number of genetic studies and by many human diseases that involve the disruption of lipid metabolic enzymes and pathways. Examples of such diseases include cancer, diabetes, as well as neurodegenerative and infectious diseases. So far, the explosion of information in the fields of genomics and proteomics has not been matched by a corresponding advancement of knowledge in the field of lipids, which is largely due to the complexity of lipids and the lack of powerful tools for their analysis. Novel analytical approaches--in particular, liquid chromatography and mass spectrometry--for systems-level analysis of lipids and their interacting partners (lipidomics) now make this field a promising area of biomedical research, with a variety of applications in drug and biomarker development.read more
Citations
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Journal ArticleDOI
Practical metabolomics in drug discovery
TL;DR: This review will focus on the practical aspects of metabolomics studies as they have been applied to the study of mammalian biological systems, specifically targeted to the steps of experimental design with regard to sample preparation, sample analysis and data analysis of both polar and non-polar metabolites.
Journal ArticleDOI
Relative Quantification of Phospholipids Based on Isotope-Labeled Methylation by Nanoflow Ultrahigh Performance Liquid Chromatography–Tandem Mass Spectrometry: Enhancement in Cardiolipin Profiling
TL;DR: The study highlighted the applicability of targeted quantification by the ILM method in lipidomic analysis and demonstrated an improvement in the detection of less abundant anionic PLs.
Journal ArticleDOI
The synthesis of 1,3-diamidophospholipids
TL;DR: A straightforward synthesis of a small library of 1,3-diamidophospholipids is presented using readily available, cheap reagents and introducing a simple phosphoramidate protecting group strategy.
Journal ArticleDOI
Discovering novel brain lipids by liquid chromatography/tandem mass spectrometry.
TL;DR: The identification and characterization of two classes of novel lipids from the brain are reviewed here: N-acyl phosphatidylserine and dolichoic acid, which benefited from the use of efficient lipid fractionation and separation techniques and highly sensitive, high-resolution tandem MS.
Journal ArticleDOI
Automated lipid A structure assignment from hierarchical tandem mass spectrometry data.
Ying S. Ting,Scott A. Shaffer,Scott A. Shaffer,Jace W. Jones,Wailap Victor Ng,Robert K. Ernst,David R. Goodlett +6 more
TL;DR: H hierarchical tandem mass spectrometry (HiTMS) algorithm aids expert interpretation of lipid A MSn data by providing the analyst with a set of candidate structures that may then be confirmed or rejected, and deciphers the signature ions and neutral losses of MSn spectra.
References
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Journal ArticleDOI
A rapid method of total lipid extraction and purification.
E. G. Bligh,W. J. Dyer +1 more
TL;DR: The lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials that has been applied to fish muscle and may easily be adapted to use with other tissues.
Journal ArticleDOI
Functional rafts in cell membranes
Kai Simons,Elina Ikonen +1 more
TL;DR: A new aspect of cell membrane structure is presented, based on the dynamic clustering of sphingolipids and cholesterol to form rafts that move within the fluid bilayer that function as platforms for the attachment of proteins when membranes are moved around inside the cell and during signal transduction.
Journal ArticleDOI
Toll-like receptor signalling
Shizuo Akira,Kiyoshi Takeda +1 more
TL;DR: Rapid progress that has recently improved the understanding of the molecular mechanisms that mediate TLR signalling is reviewed.
Journal ArticleDOI
Defective LPS Signaling in C3H/HeJ and C57BL/10ScCr Mice: Mutations in Tlr4 Gene
Alexander Poltorak,Xiaolong He,Irina Smirnova,Mu Ya Liu,Christophe Van Huffel,Xin Du,Dale Birdwell,E. Alejos,M. Silva,Chris Galanos,Marina Freudenberg,Paola Ricciardi-Castagnoli,Betsy Layton,Bruce Beutler +13 more
TL;DR: The mammalian Tlr4 protein has been adapted primarily to subserve the recognition of LPS and presumably transduces the LPS signal across the plasma membrane.
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