Journal ArticleDOI
The emerging field of lipidomics
TLDR
Novel analytical approaches — in particular, liquid chromatography and mass spectrometry — for systems-level analysis of lipids and their interacting partners (lipidomics) now make this field a promising area of biomedical research, with a variety of applications in drug and biomarker development.Abstract:
The crucial role of lipids in cell, tissue and organ physiology is demonstrated by a large number of genetic studies and by many human diseases that involve the disruption of lipid metabolic enzymes and pathways. Examples of such diseases include cancer, diabetes, as well as neurodegenerative and infectious diseases. So far, the explosion of information in the fields of genomics and proteomics has not been matched by a corresponding advancement of knowledge in the field of lipids, which is largely due to the complexity of lipids and the lack of powerful tools for their analysis. Novel analytical approaches--in particular, liquid chromatography and mass spectrometry--for systems-level analysis of lipids and their interacting partners (lipidomics) now make this field a promising area of biomedical research, with a variety of applications in drug and biomarker development.read more
Citations
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Journal ArticleDOI
Omics sciences for systems biology in Alzheimer's disease: State-of-the-art of the evidence
Harald Hampel,Robert Nisticò,Nicholas T. Seyfried,Allan I. Levey,Erica Modeste,Pablo Lemercier,Filippo Baldacci,Nicola Toschi,Francesco Garaci,George Perry,Enzo Emanuele,Pedro L. Valenzuela,Alejandro Lucia,Andrea Urbani,Giulia Maria Sancesario,Mark Mapstone,Massimo Corbo,Andrea Vergallo,Simone Lista +18 more
TL;DR: In this article, a holistic, systems-level approach is needed to fully capture Alzheimer's disease multi-faceted pathophysiology, including proteostasis (amyloid-β and tau), synaptic homeostasis, inflammatory and immune responses, lipid and energy metabolism, oxidative stress.
Computational Tools for the Untargeted Assignment of FT-MS Metabolomics Datasets
TL;DR: A set of tools were developed to assign isotoperesolved molecular formulas to observed spectral features in an untargeted manner without a database of expected metabolites, and a sub-population of nonsmall cell lung cancer samples with a significantly increased abundance in sterol lipids was revealed.
Book ChapterDOI
Analytical Tools for Lipid Assessment in Biological Assays
TL;DR: This chapter aims to present and discuss different tools available for the study of lipids and their main applications in biological assays and the advantages/disadvantages of each technique.
Journal ArticleDOI
Ultrasensitive two-dimensional material-based MCF-7 cancer cell sensor driven by perturbation processes
Sophia S. Y. Chan,Denise Lee,Maria Prisca Meivita,Lunna Li,Yaw Sing Tan,Natasa Bajalovic,Desmond Loke,Desmond Loke +7 more
TL;DR: In this paper, a direct current (DC) resistance sensor based on two-dimensional molybdenum disulfide (MoS2) nanosheets was developed to detect cancer cells.
Journal ArticleDOI
A Photosensitized Singlet Oxygen (1O2) Toolbox for Bio-Organic Applications: Tailoring 1O2 Generation for DNA and Protein Labelling, Targeting and Biosensing
TL;DR: The aim of this review is to highlight the versatility of 1O2, discussing the main bioorganic applications reported over the past decades, which rely on its production, including protein and DNA labelling, cross-linking and biosensing.
References
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Journal ArticleDOI
A rapid method of total lipid extraction and purification.
E. G. Bligh,W. J. Dyer +1 more
TL;DR: The lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials that has been applied to fish muscle and may easily be adapted to use with other tissues.
Journal ArticleDOI
Functional rafts in cell membranes
Kai Simons,Elina Ikonen +1 more
TL;DR: A new aspect of cell membrane structure is presented, based on the dynamic clustering of sphingolipids and cholesterol to form rafts that move within the fluid bilayer that function as platforms for the attachment of proteins when membranes are moved around inside the cell and during signal transduction.
Journal ArticleDOI
Toll-like receptor signalling
Shizuo Akira,Kiyoshi Takeda +1 more
TL;DR: Rapid progress that has recently improved the understanding of the molecular mechanisms that mediate TLR signalling is reviewed.
Journal ArticleDOI
Defective LPS Signaling in C3H/HeJ and C57BL/10ScCr Mice: Mutations in Tlr4 Gene
Alexander Poltorak,Xiaolong He,Irina Smirnova,Mu Ya Liu,Christophe Van Huffel,Xin Du,Dale Birdwell,E. Alejos,M. Silva,Chris Galanos,Marina Freudenberg,Paola Ricciardi-Castagnoli,Betsy Layton,Bruce Beutler +13 more
TL;DR: The mammalian Tlr4 protein has been adapted primarily to subserve the recognition of LPS and presumably transduces the LPS signal across the plasma membrane.
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