Journal ArticleDOI
The emerging field of lipidomics
TLDR
Novel analytical approaches — in particular, liquid chromatography and mass spectrometry — for systems-level analysis of lipids and their interacting partners (lipidomics) now make this field a promising area of biomedical research, with a variety of applications in drug and biomarker development.Abstract:
The crucial role of lipids in cell, tissue and organ physiology is demonstrated by a large number of genetic studies and by many human diseases that involve the disruption of lipid metabolic enzymes and pathways. Examples of such diseases include cancer, diabetes, as well as neurodegenerative and infectious diseases. So far, the explosion of information in the fields of genomics and proteomics has not been matched by a corresponding advancement of knowledge in the field of lipids, which is largely due to the complexity of lipids and the lack of powerful tools for their analysis. Novel analytical approaches--in particular, liquid chromatography and mass spectrometry--for systems-level analysis of lipids and their interacting partners (lipidomics) now make this field a promising area of biomedical research, with a variety of applications in drug and biomarker development.read more
Citations
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Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics
TL;DR: In this paper, the methyl-tert-butyl ether (MTBE) extraction method was used for shotgun profiling of lipid species, and the results showed that the MTBE protocol delivered similar or better recoveries of species of most major lipid classes compared with the "gold-standard" Folch or Bligh and Dyer recipes.
Journal ArticleDOI
The Human Serum Metabolome
Nikolaos Psychogios,David Hau,Jun Peng,An Chi Guo,Rupasri Mandal,Souhaila Bouatra,Igor Sinelnikov,Ramanarayan Krishnamurthy,Roman Eisner,Bijaya Gautam,Nelson Young,Jianguo Xia,Craig Knox,Edison Dong,Paul Huang,Zsuzsanna Hollander,Theresa L. Pedersen,Steven R. Smith,Fiona Bamforth,Russell Greiner,Bruce M. McManus,John W. Newman,Theodore L. Goodfriend,David S. Wishart,David S. Wishart +24 more
TL;DR: This work has combined targeted and non-targeted NMR, GC-MS and LC-MS methods with computer-aided literature mining to identify and quantify a comprehensive, if not absolutely complete, set of metabolites commonly detected and quantified (with today's technology) in the human serum metabolome.
Journal ArticleDOI
Global analysis of the yeast lipidome by quantitative shotgun mass spectrometry
Christer S. Ejsing,Julio L. Sampaio,Vineeth Surendranath,Eva Duchoslav,Kim Ekroos,Robin W. Klemm,Kai Simons,Andrej Shevchenko +7 more
TL;DR: Comparative lipidomics demonstrated that growth temperature and defects in lipid biosynthesis induce ripple effects throughout the molecular composition of the yeast lipidome, establishing shotgun lipidomics as a powerful platform for complementing biochemical studies and other systems-level approaches.
Journal ArticleDOI
ABC transporters in cancer: more than just drug efflux pumps.
TL;DR: It is proposed that delineating the precise roles of these transporters in tumorigenesis and treatment response will be important for the development of more effective targeted therapies.
Journal ArticleDOI
The model organism as a system: integrating 'omics' data sets.
TL;DR: Researchers are rising to the challenge by using omics data integration to address fundamental biological questions that would increase the understanding of systems as a whole.
References
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Journal ArticleDOI
MmpL8 is required for sulfolipid-1 biosynthesis and Mycobacterium tuberculosis virulence
Scott E. Converse,Joseph D. Mougous,Michael D. Leavell,Julie A. Leary,Carolyn R. Bertozzi,Jeffery S. Cox +5 more
TL;DR: It is shown that MmpL8, a member of a large family of predicted lipid transporters in M. tuberculosis, is required for SL-1 production and that mMPL8 mutants are attenuated for growth in a mouse model of tuberculosis.
Journal ArticleDOI
Pleckstrin homology domains: not just for phosphoinositides
TL;DR: Findings in yeast indicate that small GTPases such as ARF family proteins play a role in defining PH domain localization, and herald a change in the perspective of PH domain function, which will be significantly more diverse than previously supposed.
Journal ArticleDOI
Defects in Cell Growth Regulation by C18:0-Ceramide and Longevity Assurance Gene 1 in Human Head and Neck Squamous Cell Carcinomas *
Serap Koybasi,Can E. Senkal,Kamala P. Sundararaj,Stefka D. Spassieva,Jacek Bielawski,Walid Osta,Terry A. Day,James C. Jiang,S. Michal Jazwinski,Yusuf A. Hannun,Lina M. Obeid,Besim Ogretmen +11 more
TL;DR: A biological role for LAG1 and C18-ceramide in the regulation of growth of HNSCC is demonstrated, for the first time, in a liquid chromatography/mass spectroscopy study.
Journal ArticleDOI
Automated quantitative analysis of complex lipidomes by liquid chromatography/mass spectrometry.
TL;DR: This work has developed an automated method that allows unattended identification and quantification of lipid molecular species of all the major lipid classes from a two-dimensional chromatographic/mass spectrometric data set and makes high-throughput lipidomics feasible in biology, biotechnology, and medicine.
Journal ArticleDOI
Toward fingerprinting cellular lipidomes directly from biological samples by two-dimensional electrospray ionization mass spectrometry.
TL;DR: A novel two-dimensional electrospray ionization mass spectrometric approach is presented to fingerprint most of the major and many of the minor lipid classes in the hepatic cellular lipidome, which collectively represent >80% of the total lipid mass, directly from their chloroform extracts.
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