Journal ArticleDOI
The emerging field of lipidomics
TLDR
Novel analytical approaches — in particular, liquid chromatography and mass spectrometry — for systems-level analysis of lipids and their interacting partners (lipidomics) now make this field a promising area of biomedical research, with a variety of applications in drug and biomarker development.Abstract:
The crucial role of lipids in cell, tissue and organ physiology is demonstrated by a large number of genetic studies and by many human diseases that involve the disruption of lipid metabolic enzymes and pathways. Examples of such diseases include cancer, diabetes, as well as neurodegenerative and infectious diseases. So far, the explosion of information in the fields of genomics and proteomics has not been matched by a corresponding advancement of knowledge in the field of lipids, which is largely due to the complexity of lipids and the lack of powerful tools for their analysis. Novel analytical approaches--in particular, liquid chromatography and mass spectrometry--for systems-level analysis of lipids and their interacting partners (lipidomics) now make this field a promising area of biomedical research, with a variety of applications in drug and biomarker development.read more
Citations
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Journal ArticleDOI
Human carboxylesterases and their role in xenobiotic and endobiotic metabolism.
Matthew K. Ross,J. Allen Crow +1 more
TL;DR: The hydrolytic activity of individual HLMs toward bioresmethrin and procaine did not correlate with the protein content of hCE1 and hCE2, so the mere abundance of CE proteins is not a good predictor of CE activity in HLMs.
Journal ArticleDOI
Advances in Lipidomics for Cancer Biomarkers Discovery
F. Perrotti,Consuelo Rosa,Ilaria Cicalini,Paolo Sacchetta,Piero Del Boccio,Domenico Genovesi,Damiana Pieragostino +6 more
TL;DR: The novel lipidomic biomarkers are reviewed in an effort to underline their role in diagnosis, in prognostic characterization and in prediction of therapeutic outcomes, and a lipidomic investigation through mass spectrometry highlights new insights on molecular mechanisms underlying cancer disease.
Journal ArticleDOI
Lipidomics profiling by high-resolution LC-MS and high-energy collisional dissociation fragmentation: focus on characterization of mitochondrial cardiolipins and monolysocardiolipins.
Susan S. Bird,Vasant R. Marur,Vasant R. Marur,Matthew J. Sniatynski,Matthew J. Sniatynski,Heather K. Greenberg,Heather K. Greenberg,Bruce S. Kristal,Bruce S. Kristal +8 more
TL;DR: The developed lipid profiling method was applied to mitochondrial samples from an animal study related to the linkages between diet, mitochondrial function, and disease and identified 28 unique CL species and two monolysocardiolipin species that are often associated with mitochondrial stress and dysfunction.
Journal ArticleDOI
Protocol: a fast, comprehensive and reproducible one-step extraction method for the rapid preparation of polar and semi-polar metabolites, lipids, proteins, starch and cell wall polymers from a single sample
TL;DR: The described extraction protocol provides a simple and straightforward method for the efficient extraction of lipids, metabolites and proteins from minute amounts of a single sample, enabling the targeted but also untargeted high-throughput analyses of diverse biological tissues and samples.
Journal ArticleDOI
Lipidomic profiling of biological tissues using off-line two-dimensional high-performance liquid chromatography–mass spectrometry
TL;DR: A new method for the characterization of the lipid composition in various tissues, using off-line two-dimensional coupling of hydrophilic interaction liquid chromatography and reversed-phase (RP) high-performance liquid Chromatography coupled to electrospray ionization (ESI) and atmospheric pressure chemical ionizations (APCI) mass spectrometry.
References
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E. G. Bligh,W. J. Dyer +1 more
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Functional rafts in cell membranes
Kai Simons,Elina Ikonen +1 more
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TL;DR: The mammalian Tlr4 protein has been adapted primarily to subserve the recognition of LPS and presumably transduces the LPS signal across the plasma membrane.
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