Journal ArticleDOI
The emerging field of lipidomics
TLDR
Novel analytical approaches — in particular, liquid chromatography and mass spectrometry — for systems-level analysis of lipids and their interacting partners (lipidomics) now make this field a promising area of biomedical research, with a variety of applications in drug and biomarker development.Abstract:
The crucial role of lipids in cell, tissue and organ physiology is demonstrated by a large number of genetic studies and by many human diseases that involve the disruption of lipid metabolic enzymes and pathways. Examples of such diseases include cancer, diabetes, as well as neurodegenerative and infectious diseases. So far, the explosion of information in the fields of genomics and proteomics has not been matched by a corresponding advancement of knowledge in the field of lipids, which is largely due to the complexity of lipids and the lack of powerful tools for their analysis. Novel analytical approaches--in particular, liquid chromatography and mass spectrometry--for systems-level analysis of lipids and their interacting partners (lipidomics) now make this field a promising area of biomedical research, with a variety of applications in drug and biomarker development.read more
Citations
More filters
Journal ArticleDOI
Lipidomics as an emerging tool to predict endometrial receptivity
TL;DR: The results currently available indicate that prostaglandins E(2) and F(2α) are particularly abundant during the window of implantation and that they might serve to nurse the blastocyst at the time of embryo implantation; they may also serve as important biomarkers to define the receptive phase of the endometrium.
Journal ArticleDOI
Lipidomics in the study of lipid metabolism: Current perspectives in the omic sciences.
Ana Emília Holanda Rolim,Ricardo Henrique-Araújo,Eduardo Gomes Ferraz,Fátima Karoline Araújo Alves Dultra,Luzimar Gonzaga Fernandez +4 more
TL;DR: The advances in systems biology and in the development of new technological tools in analysis, among which, metabolomics, and more specifically, lipidomics, have made it possible to investigate the structural and functional complexity of lipids in biological systems.
Journal ArticleDOI
Plasma lipidomic profiling method based on ultrasound extraction and liquid chromatography mass spectrometry.
Consuelo Pizarro,Irene Arenzana-Rámila,N. Pérez-del-Notario,Patricia Pérez-Matute,José-María González-Sáiz +4 more
TL;DR: This study presents a new method for exhaustive lipid fingerprinting of human blood plasma samples based on the employment of methyl tert-butyl ether and ultrasound (US) energy combined with liquid chromatography-electrospray ionization quadrupole-time-of-flight mass spectrometry (LC-ESIqToF-MS).
Journal ArticleDOI
Label-free biosensing with lipid-functionalized gold nanorods.
TL;DR: The fabrication of a label-free mass spectrometry and optical detection-based biosensor platform for the detection of low-abundance lipophilic analytes in complex mixtures is described and the effectiveness is demonstrated.
Journal ArticleDOI
Direct ionization of biological tissue for mass spectrometric analysis.
TL;DR: It is reported that both plant and animal tissues can be directly ionized and analyzed by mass spectrometry under ambient conditions and allows observation of compounds that cannot be detected by other ionization techniques.
References
More filters
Journal ArticleDOI
A rapid method of total lipid extraction and purification.
E. G. Bligh,W. J. Dyer +1 more
TL;DR: The lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials that has been applied to fish muscle and may easily be adapted to use with other tissues.
Journal ArticleDOI
Functional rafts in cell membranes
Kai Simons,Elina Ikonen +1 more
TL;DR: A new aspect of cell membrane structure is presented, based on the dynamic clustering of sphingolipids and cholesterol to form rafts that move within the fluid bilayer that function as platforms for the attachment of proteins when membranes are moved around inside the cell and during signal transduction.
Journal ArticleDOI
Toll-like receptor signalling
Shizuo Akira,Kiyoshi Takeda +1 more
TL;DR: Rapid progress that has recently improved the understanding of the molecular mechanisms that mediate TLR signalling is reviewed.
Journal ArticleDOI
Defective LPS Signaling in C3H/HeJ and C57BL/10ScCr Mice: Mutations in Tlr4 Gene
Alexander Poltorak,Xiaolong He,Irina Smirnova,Mu Ya Liu,Christophe Van Huffel,Xin Du,Dale Birdwell,E. Alejos,M. Silva,Chris Galanos,Marina Freudenberg,Paola Ricciardi-Castagnoli,Betsy Layton,Bruce Beutler +13 more
TL;DR: The mammalian Tlr4 protein has been adapted primarily to subserve the recognition of LPS and presumably transduces the LPS signal across the plasma membrane.
Related Papers (5)
Shotgun lipidomics: Electrospray ionization mass spectrometric analysis and quantitation of cellular lipidomes directly from crude extracts of biological samples
Xianlin Han,Richard W. Gross +1 more
A comprehensive classification system for lipids
Eoin Fahy,Shankar Subramaniam,H. Alex Brown,Christopher K. Glass,Alfred H. Merrill,Robert C. Murphy,Christian R. H. Raetz,David W. Russell,Yousuke Seyama,Walter A. Shaw,Takao Shimizu,Friedrich Spener,Gerrit van Meer,Michael S. VanNieuwenhze,Stephen H. White,Joseph L. Witztum,Edward A. Dennis +16 more