Chitosan Wound Dressings Incorporating Exosomes Derived from MicroRNA-126-Overexpressing Synovium Mesenchymal Stem Cells Provide Sustained Release of Exosomes and Heal Full-Thickness Skin Defects in a Diabetic Rat Model.
TLDR
The first evidence of the potential of SMSC‐126‐Exos in treating cutaneous wounds is provided and indicates that modifying the cells—for example, by gene overexpression—and using the exosomes derived from these modified cells provides a potential drug delivery system and could have infinite possibilities for future therapy.Abstract:
There is a need to find better strategies to promote wound healing, especially of chronic wounds, which remain a challenge We found that synovium mesenchymal stem cells (SMSCs) have the ability to strongly promote cell proliferation of fibroblasts; however, they are ineffective at promoting angiogenesis Using gene overexpression technology, we overexpressed microRNA-126-3p (miR-126-3p) and transferred the angiogenic ability of endothelial progenitor cells to SMSCs, promoting angiogenesis We tested a therapeutic strategy involving controlled-release exosomes derived from miR-126-3p-overexpressing SMSCs combined with chitosan Our in vitro results showed that exosomes derived from miR-126-3p-overexpressing SMSCs (SMSC-126-Exos) stimulated the proliferation of human dermal fibroblasts and human dermal microvascular endothelial cells (HMEC-1) in a dose-dependent manner Furthermore, SMSC-126-Exos also promoted migration and tube formation of HMEC-1 Testing this system in a diabetic rat model, we found that this approach resulted in accelerated re-epithelialization, activated angiogenesis, and promotion of collagen maturity in vivo These data provide the first evidence of the potential of SMSC-126-Exos in treating cutaneous wounds and indicate that modifying the cells-for example, by gene overexpression-and using the exosomes derived from these modified cells provides a potential drug delivery system and could have infinite possibilities for future therapy Stem Cells Translational Medicine 2017;6:736-747read more
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Immune Regulation of Skin Wound Healing: Mechanisms and Novel Therapeutic Targets.
TL;DR: The pathophysiology of both acute and chronic wounds and possible strategies to control the immune system to accelerate chronic wound closure and promote skin regeneration (scar-less healing) of acute wounds are discussed.
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Emerging and innovative approaches for wound healing and skin regeneration: Current status and advances
TL;DR: This review reviewed various design considerations for skin repair and impact of several smart technologies viz., in situ 3D printing, portable bioprinters, electrosprayers and in situ forming hydrogels that have significantly improved wound healing and skin therapeutics.
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Efficient Angiogenesis-Based Diabetic Wound Healing/Skin Reconstruction through Bioactive Antibacterial Adhesive Ultraviolet Shielding Nanodressing with Exosome Release.
Min Wang,Chenggui Wang,Chenggui Wang,Mi Chen,Yuewei Xi,Wei Cheng,Cong Mao,Tianzhen Xu,Xingxing Zhang,Cai Lin,Weiyang Gao,Yi Guo,Bo Lei +12 more
TL;DR: An injectable adhesive thermosensitive multifunctional polysaccharide-based dressing with sustained pH-responsive exosomes release with great potential in achieving satisfactory healing in diabetic and other vascular-impaired related wounds was fabricated.
Journal ArticleDOI
Exosomes from adipose-derived stem cells overexpressing Nrf2 accelerate cutaneous wound healing by promoting vascularization in a diabetic foot ulcer rat model
TL;DR: The data suggest that exosomes from ADSCs can potentially promote wound healing, particularly when overexpressing Nrf2 and therefore that the transplantation of exosome may be suitable for clinical application in the treatment of DFUs.
Journal ArticleDOI
Enhanced Therapeutic Effects of Mesenchymal Stem Cell-Derived Exosomes with an Injectable Hydrogel for Hindlimb Ischemia Treatment
Kaiyue Zhang,Xiangnan Zhao,Xiaoniao Chen,Yongzhen Wei,Wei Du,Yuebing Wang,Linan Liu,Weian Zhao,Zhibo Han,Deling Kong,Qiang Zhao,Zhikun Guo,Zhongchao Han,Na Liu,Fengxia Ma,Zongjin Li +15 more
TL;DR: It is demonstrated that chitosan hydrogel could enhance the retention and stability of exosomes and further augment the therapeutic effects for hindlimb ischemia as revealed by firefly luciferase imaging of angiogenesis.
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