Organization and Ca2+ Regulation of Adenylyl Cyclases in cAMP Microdomains
TLDR
The regulation of many of the ACs by the ubiquitous second messenger Ca(2+) provides an overarching mechanism for integrating the activities of these two major signaling systems, and cAMP will exhibit distinct kinetics in discrete cellular domains.Abstract:
The adenylyl cyclases are variously regulated by G protein subunits, a number of serine/threonine and tyrosine protein kinases, and Ca2+. In some physiological situations, this regulation can be re...read more
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Dissertation
Smooth muscle cell proliferation and endothelin pathway in pulmonary arterial hypertension : comparative effects of current and putative therapeutic agents
TL;DR: In chapter 5, the antiproliferative effects of an already established anti-cancer drug ispinesib is evaluated and established a rationale for investigating this agent in PAH settings.
Journal ArticleDOI
Themed section: molecular pharmacology of GPCRs
TL;DR: This themed issue of the British Journal of Pharmacology contains a series of papers that cover recent developments and identify approaches that may help determine future directions of GPCRs.
Dissertation
Role of CFTR and chloride/bicarbonate exchangers in airway epithelial bicarbonate secretion
TL;DR: Findings provide new insights into the signalling pathways that regulate both the apical and basolateral anion exchangers in Calu-3 cells and help define their respective roles in airway HCO3 secretion.
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Assay for Detecting Gαi-Mediated Decreases in cAMP in Living Cells:
Paul Tewson,Scott Martinka,Nathan C. Shaner,Catherine H. Berlot,Anne Marie Quinn,Thomas E. Hughes +5 more
TL;DR: The green fluorescent cADDis sensor is applied to the detection of Gαi-mediated inhibition of adenylyl cyclase activity and is validated and optimized using several known G αi-coupled receptors and agonists and reported robust Z′ statistics and consistent EC50 responses.
Dissertation
Functional characterisation of phosphodiesterase 4D7 in prostate cancer
TL;DR: Significant changes in cAMP signalling during PC progression are found by studying the expression of cAMP PDE isoforms, with the aim of identifying a novel PC biomarker, as the current standard biomarker (PSA) is not disease-specific and leads to much over-diagnosis and over-treatment of otherwise non-life threatening prostate tumours.
References
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Journal ArticleDOI
Lipid rafts and signal transduction
Kai Simons,Derek Toomre +1 more
TL;DR: It is now becoming clear that lipid micro-environments on the cell surface — known as lipid rafts — also take part in this process of signalling transduction, where protein–protein interactions result in the activation of signalling cascades.
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Store-operated calcium channels.
Anant B. Parekh,James W. Putney +1 more
TL;DR: The key electrophysiological features of I(CRAC) and other store-operated Ca(2+) currents and how they are regulated are described, and recent advances that have shed insight into the molecular mechanisms involved in this ubiquitous and vital Ca( 2+) entry pathway are considered.
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Cyclic nucleotide phosphodiesterases: functional implications of multiple isoforms
TL;DR: This article reviews many of the more important aspects about the structure, cellular localization, and regulation of each family of phosphodiesterases and places particular emphasis on new information obtained in the last few years about how differential expression andregulation of individual phosphodiesters relate to their function(s) in the body.
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The role of hormone receptors and GTP-regulatory proteins in membrane transduction
TL;DR: Cell membrane receptors for hormones and neurotransmitters form oligomeric complexes with GTP-regulatory proteins and inhibit the latter from reacting with G TP, and this theory may apply generally to membrane signal transduction involving surface receptors.
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Lipid Rafts: Elusive or Illusive?
TL;DR: There has been considerable recent interest in the possibility that the plasma membrane contains lipid "rafts," microdomains enriched in cholesterol and sphingolipids, and it seems that a definitive proof of raft existence has yet to be obtained.
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