Ministry of Agriculture

About: Ministry of Agriculture is a government organization based out in Rio de Janeiro, Brazil. It is known for research contribution in the topics: Biology & Chemistry. The organization has 1153 authors who have published 1189 publications receiving 14442 citations.

Genotyping of Toxoplasma gondii strains detected in pork sausage

Abstract: DNA was extracted from 70 pork sausage samples obtained from 55 commercial establishments inthe city of Botucatu, Sao Paulo, Brazil. Nested-polymerase chain reaction with primers specific for Toxoplasma gondii SAG2 locus was used and detected the parasite in 19 (27.14%) sausage samples.Digestion of amplicons with restriction enzymes HhaI and Sau3AI showed that 14 (73.68%) samplescontained parasites genotyped as Type I, and 5 (26.32%) as Type III. Key words: Toxoplasma gondii ; SAG2 genotyping; food-borne pathogen; sausage; swine. * Universidade Paranaense (UNIPAR). Praca Mascarenhas de Moraes, s/n, 87.502-210, Umuarama, Parana, Brazil.** Laboratorio Regional de Apoio Animal, Ministerio da Agricultura, Pecuaria e Abastecimento. Rua Raul Ferrari, s/n, 13.094-430, Campinas, Sao Paulo, Brazil.***Nucleo de Pesquisa em Zoonoses (NUPEZO), Departamento de Higiene Veterinaria e Saude Publica (DHVSP),Faculdade de Medicina Veterinaria e Zootecnia (FMVZ), Universidade Estadual Paulista (UNESP), CampusBotucatu. Distrito de Rubiao Jr., s/n, 18.610-000, Botucatu, Sao Paulo, Brazil.Corresponding author: Tel: 55 44 36225126 - fax: 55 44 36225126 - e-mail address: silva.av@uol.com.br

Differentiation of rum and Brazilian artisan cachaça via electrospray ionization mass spectrometry fingerprinting.

Abstract: Rum and cachaca are sugarcane distillates produced on large scales and of similar composition, and their differentiation is currently a subject of commercial dispute and a challenging analytical task. We have investigated the ability of direct-infusion electrospray ionization mass spectrometry in the negative ion mode, i.e. ESI(-)-MS, to distinguish between samples of these distillates. ESI(-)-MS fingerprints were collected for some samples of Brazilian artisan cachaca, aged in two types of wooden casks, i. e. amburana (Amburana cearensis) and jequitiba (Cariniana estrellensis), and of commercial rum. The mass spectra were found to be very distinctive, showing sets of diagnostic ions for each type of sample, i. e. (1) cachaca aged in amburana (m/z 271, 313, 377) and jequitiba (m/z 171, 255, 455) casks; and (2) commercial rum (m/z 89, 97, 179, 255, 283). When applied to the ESI(-)-MS data, principal component analysis and hierarchical cluster analysis split rum and cachaca samples into well-defined groups. Moreover, the two types of cachaca samples aged in wooden casks of amburana or jequitiba were also split into two distinct groups. Direct-infusion ESI(-)-MS can therefore be potentially applied to the rapid, simple, and accurate differentiation of these commercially important sugarcane distillates.

Neuere Beiträge zur Kenntnis der Taktosole

Abstract: In einem 4 Jahre alten Aluminiumhydroxol wurde bei Untersuchung in polarisiertem Licht ein Sediment von einem neuen Taktosol aufgefunden, das auch bei Schlierenbeleuchtung gut sichtbar ist. Die Struktur der Konfigurationen entspricht der Mechanik der nematischen Phasen. Die Form eines einfachen Tropfens umgeben von Ataktosol („Taktoide“) wurde eingehend betrachtet.