Ministry of Agriculture

About: Ministry of Agriculture is a government organization based out in Rio de Janeiro, Brazil. It is known for research contribution in the topics: Biology & Chemistry. The organization has 1153 authors who have published 1189 publications receiving 14442 citations.

Óleos essenciais da Amazonia contendo linalol

Abstract: Sinopse A continuidade da producao de oleo essencial de pau-rosa na Amazonia, nao esta assegurada, em virtude das dificuldades resultantes da propagacao e do crescimento lento da Aniba duckei Kosterm. Com o proposito de resolver o problema se estabeleceram duas linhas de pesquisas: 1. a analise (incluindo variacoes periodicas da composicao) do oleo das folhas de pau-rosa (rendimento de 2,0-2,5%) obtido da Aniba duckei Kosterm. (Lauraceae). 2. um estudo das plantas comuns na Amazonia, quanto a seu teor de linalol, que levou a descoberta do oleo das folhas (rendimento 0,8%) obtido de Croton cajucara Benth. (Euphorbiaceae), contendo mais de 66% de linalol.

β-lactam antibiotics residues analysis in bovine milk by LC-ESI-MS/MS: a simple and fast liquid–liquid extraction method

Abstract: This study presents the development and validation of a simple method for the detection and quantification of six β-lactam antibiotics residues (ceftiofur, penicillin G, penicillin V, oxacillin, cloxacillin and dicloxacillin) in bovine milk using a fast liquid-liquid extraction (LLE) for sample preparation, followed by liquid chromatography-electrospray-tandem mass spectrometry (LC-MS/MS). LLE consisted of the addition of acetonitrile to the sample, followed by addition of sodium chloride, centrifugation and direct injection of an aliquot into the LC-MS/MS system. Separation was performed in a C(18) column, using acetonitrile and water, both with 0.1% of formic acid, as mobile phase. Method validation was performed according to the criteria of Commission Decision 2002/657/EC. Limits of detection ranged from 0.4 (penicillin G and penicillin V) to 10.0 ng ml(-1) (ceftiofur), and linearity was achieved. The decision limit (CCα), detection capability (CCβ), accuracy, inter- and intra-day repeatability of the method are reported.

Mormo em eqüídeos nos Estados de Pernambuco e Alagoas

Abstract: Based on clinical-pathological, epidemiological and serological aspects, as well as by the isolation of Burkholderia mallei, foci of glanders were diagnosed in equids of the "Zona da Mata" in the states of Pernambuco and Alagoas. Clinically there was hyperthermia, noisy respiration, loss of appetite, progressive loss of weight leading to emaciation, nasal congestion and muco-purulent discharge, erosions, ulcers and scars of the nasal mucosa. The size of submaxillary and cervical lymphonodes was increased, which contained abscesses and fistules exudating purulent material. In the skin, accompanying the lymph vessels, there were firm nodules and abscesses which in chronic cases fistulated and ulcerated leaving stellar scars. The histopathology of the nodules at various sites, from the horses as well as from the guinea-pigs used for the Strauss test, showed granulomatous lesions and pyogranulomas. The results of this study showed that glanders in the region was emerging and further sero-epidemiological studies are urgent for the eradication of the disease.