Ministry of Agriculture

About: Ministry of Agriculture is a government organization based out in Rio de Janeiro, Brazil. It is known for research contribution in the topics: Biology & Chemistry. The organization has 1153 authors who have published 1189 publications receiving 14442 citations.

Clean-up Procedure Development and Method Validation for Pesticide Residues Analysis in Carrots

Abstract: In this work, a method was developed, validated, and applied to determination of ten pesticide residues (trifluralin, carbofuran, chlorothalonil, pendimethalin, captan, tebuconazole, pyraclostrobin, difenoconazole, deltamethrin, and azoxystrobin), from seven distinct classes, in carrot samples, employing a QuEChERS procedure for the analyte extraction and gas chromatography coupled to mass spectrometry (GC-MS) for quantification. The dispersive clean-up step was optimized using different sorbents at different concentrations and mixings. The method proved to be efficient and robust, showing recoveries in the range 72–117%, and RSD ranging from 4.9 to 16.6% (except for chlorothalonil and captan) at fortification levels of 45, 90, 180, and 450 μg kg−1 with seven replicates analyzed for each level (n = 28). Chlorothalonil and captan could not be validated due to analyte stability problems. Detection limits were between 15 and 45 μg kg−1, and the quantification limits were between 45 and 90 μg kg−1. The method was successfully applied to real samples.

D-loop haplotype diversity in Brazilian horse breeds.

Abstract: The first horses were brought to Brazil by the colonizers after 1534. Over the centuries, these animals evolved and adapted to local environmental conditions usually unsuitable for exotic breeds, thereby originating locally adapted Brazilian breeds. The present work represents the first description of maternal genetic diversity in these horse breeds based on D-loop sequences. A D-Loop HSV-I fragment of 252 bp, from 141 horses belonging to ten Brazilian breeds / genetic groups (locally adapted and specialized breeds) were analysed. Thirty-five different haplotypes belonging to 18 haplogroups were identified with 33 polymorphic sites. Haplotype diversity (varying from 0.20 to 0.96) and nucleotide diversity (varying from 0.0039 to 0.0239) was lower for locally adapted than for specialized breeds, with the same pattern observed for FST values. Haplogroups identified in Brazilian breeds are in agreement with previous findings in South American samples. The low variability observed mainly in locally adapted breeds, indicates that, to ensure conservation of these breeds, careful reproductive management is needed. Additional genetic characterization studies are required to support accurate decision-making.

Efeito da idade de desmame no desenvolvimento e nas características de carcaça de novilhos de corte

Abstract: Avaliou-se o efeito da idade de desmame no desempenho e nas caracteristicas de carcaca de bezerros filhos de vacas cruzas zebuinas x taurinas, primiparas aos 3 anos de idade. As vacas foram mantidas durante dois invernos/primaveras, do desmame a primeira estacao de monta, em pastejo rotativo em dois grupos (ponta e rapador), em pastagens de azevem anual (Lolium multiflorum Lam) e trevo vesiculoso (Trifolium vesiculosum cv. Yuchi) e, posteriormente, durante o verao e o outono, em campo nativo. Os bezerros foram distribuidos aleatoriamente, segundo o grupo racial e o grupo de pastejo das vacas, em duas idades de desmame: desmame precoce (DP) = sete bezerros desmamados aos 70 dias de idade com 83,2 kg de PV medio; e idade convencional (DC) = dez bezerros desmamados com 180 dias de idade e 152,0 kg de PV medio. O delineamento experimental foi o completamente casualizado. Os novilhos foram abatidos aos 2 anos de idade. O grupo de manejo das vacas (ponta ou rapador) nao teve efeito sobre o desempenho e as caracteristicas de carcaca dos novilhos. Os animais do desmame convencional foram mais pesados ate 1 ano de idade (DP = 183,0 kg vs DC = 202,1 kg), mas nao diferiram dos demais quando abatidos aos 2 anos de idade (DP = 411,2 kg vs DC = 408,5 kg). As carcacas nao diferiram quanto ao acabamento, a conformacao, a classificacao "Cota Hilton" e ao rendimento. Portanto, o desmame precoce nao influenciou o desenvolvimento e as caracteristicas de carcaca ao abate aos 2 anos de idade.