A rule-based data-informed cellular consensus map of the human mononuclear phagocyte cell space
Patrick Günther,Branko Cirovic,Kevin Baßler,Kristian Händler,Matthias Becker,Charles-Antoine Dutertre,Venetia Bigley,Evan W. Newell,Matthew Collin,Florent Ginhoux,Florent Ginhoux,Andreas Schlitzer,Joachim L. Schultze,Joachim L. Schultze +13 more
TLDR
A rule-based data-informed approach to build next generation cellular consensus maps, using the human dendritic-cell and monocyte compartment in peripheral blood as an example, and providing a generalizable method for building consensus maps for the life sciences.Abstract:
Single-cell genomic techniques are opening new avenues to understand the basic units of life. Large international efforts, such as those to derive a Human Cell Atlas, are driving progress in this area; here, cellular map generation is key. To expedite the inevitable iterations of these underlying maps, we have developed a rule-based data-informed approach to build next generation cellular consensus maps. Using the human dendritic-cell and monocyte compartment in peripheral blood as an example, we performed computational integration of previous, partially overlapping maps using an approach we termed ‘backmapping’, combined with multi-color flow-cytometry and index sorting-based single-cell RNA-sequencing. Our general strategy can be applied to any atlas generation for humans and other species. Graphical Highlights Defining a consensus of the human myeloid cell compartment in peripheral blood 3 monocytes subsets, pDC, cDC1, DC2, DC3 and precursor DC make up the compartment Distinguish myeloid cell compartment from other cell spaces, e.g. the NK cell space Providing a generalizable method for building consensus maps for the life sciencesread more
Citations
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Human Monocyte Subsets and Phenotypes in Major Chronic Inflammatory Diseases
Theodore S. Kapellos,Lorenzo Bonaguro,Ioanna Gemünd,Nico Reusch,Adem Saglam,Emily R. Hinkley,Joachim L. Schultze +6 more
TL;DR: An insight is provided into the contribution of human monocytes to the progression of these diseases and their candidacy as potential therapeutic cell targets are highlighted.
Journal ArticleDOI
Single-Cell Analysis of Human Mononuclear Phagocytes Reveals Subset-Defining Markers and Identifies Circulating Inflammatory Dendritic Cells.
Charles-Antoine Dutertre,Charles-Antoine Dutertre,Etienne Becht,Sergio Erdal Irac,Sergio Erdal Irac,Ahad Khalilnezhad,Ahad Khalilnezhad,Vipin Narang,Shabnam Khalilnezhad,Pei Y. Ng,Lucas L van den Hoogen,Jing Yao Leong,Bernett Lee,Marion Chevrier,Xiao Meng Zhang,Pearly Yong,Geraldine Koh,Josephine Lum,Shanshan W. Howland,Esther Wing Hei Mok,Jinmiao Chen,Anis Larbi,Henry K.K. Tan,Tony Kiat Hon Lim,Panagiota Karagianni,Athanasios G. Tzioufas,Benoit Malleret,Benoit Malleret,Joshua Brody,Salvatore Albani,Joel A G van Roon,Timothy R D J Radstake,Evan W. Newell,Florent Ginhoux,Florent Ginhoux +34 more
TL;DR: High-dimensional single-cell protein and RNA expression data is integrated to identify distinct markers to delineate monocytes from conventional DC2 (cDC2s), further unravel the heterogeneity of DC subpopulations in health and disease and may pave the way for the identification of specific DC subset-targeting therapies.
Journal ArticleDOI
Single-cell profiling of myeloid cells in glioblastoma across species and disease stage reveals macrophage competition and specialization.
Ana Rita Pombo Antunes,Isabelle Scheyltjens,Francesca Lodi,Julie Messiaen,Asier Antoranz,Johnny Duerinck,Daliya Kancheva,Liesbet Martens,Karen De Vlaminck,Hannah Van Hove,Signe Schmidt Kjølner Hansen,Francesca Maria Bosisio,Koen Van der Borght,Steven De Vleeschouwer,Steven De Vleeschouwer,Raf Sciot,Luc Bouwens,M. Verfaillie,Niels Vandamme,Roosmarijn E. Vandenbroucke,Olivier De Wever,Yvan Saeys,Martin Guilliams,Conny Gysemans,Bart Neyns,Frederik De Smet,Diether Lambrechts,Jo A. Van Ginderachter,Kiavash Movahedi +28 more
TL;DR: This article employed single-cell RNA sequencing and CITE-seq to map the glioblastoma immune landscape in mouse tumors and in patients with newly diagnosed disease or recurrence.
Journal ArticleDOI
Human Intestinal Mononuclear Phagocytes in Health and Inflammatory Bowel Disease.
Charles Caër,Mary Jo Wick +1 more
TL;DR: The current understanding of MNP ontogeny, as well as the recently identified human intestinal MNP subsets, are reviewed, and their role in health and IBD is discussed.
Journal ArticleDOI
Monocyte Regulation in Homeostasis and Malignancy
TL;DR: In this article, the influence of cancer on the systemic alteration of monocyte subset abundance and transcriptional profiles in the circulation has been revealed, exemplified by the influence that cancer can enhance the metastatic spread of primary cancers and may offer therapeutic opportunities.
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Book
Cartography: The Ideal and Its History
TL;DR: The most influential and productive map historians of the present are: Matthew H. Edney as discussed by the authors, who has published a large number of academic articles and book reviews and is considered one of the most influential map historians in history.
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