Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
Citations
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Pushing product formation to its limit: metabolic engineering of Corynebacterium glutamicum for L-leucine overproduction.
TL;DR: Using metabolic engineering, an efficient L-leucine production strain of Corynebacterium glutamicum was developed using a feedback-resistant IMPS variant, which carries two amino acid exchanges and is highly interesting for industrial application.
Journal ArticleDOI
Lipolysis Produces Changes in the Immunoreactivity and Cell Reactivity of Very Low Density Lipoproteins
TL;DR: VLDL lipid hydrolysis was accompanied by changes in the immunoreactivity of VLDL-ApoB, which probably reflect changes inThe disposition of ApoB on the surface of V LDL, which may be related to their enhanced interaction with cells.
Journal ArticleDOI
Antiestrogens and steroid hormones: substrates of the human P-glycoprotein.
TL;DR: The results suggest that the antiestrogens and steroid hormones that are known to reverse the multidrug-resistant phenotype do so by directly interacting with Pgp, thus interfering with its anticancer drug-extruding activity.
Journal ArticleDOI
Trehalose : Protector of antioxidant enzymes or reactive oxygen species scavenger under heat stress?
Y. Luo,Wei-Min Li,Wei Wang +2 more
TL;DR: In this article, the authors examined the effects of trehalose on the activities of key antioxidant enzymes, including superoxide dismutases (SODs), ascorbate catalases (CATs), and ascarbate peroxidases (APX) from wheat (Triticum aestivum L.), and then measured the ability of Trehalose to scavenge hydrogen peroxide (H2O2) and superoxide anions (O2− ).
Book ChapterDOI
Caveolae purification and glycosylphosphatidylinositol-linked protein sorting in polarized epithelia.
TL;DR: This chapter describes the techniques used for the recombinant expression of glycosylphosphatidylinositol (GPI)-linked proteins in epithelial cell lines and the measurement of cell-surface polarity of endogenous or transfected GPI-linked proteins at steady state and during transport.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
Journal ArticleDOI
Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
Journal ArticleDOI
Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.