Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
Citations
More filters
Journal ArticleDOI
Expression of a kinase anchor protein 75 depletes type II cAMP-dependent protein kinases from the cytoplasm and sequesters the kinases in a particulate pool.
TL;DR: This work addresses previously untested propositions by overexpressing bovine AKAP75 in a human cell line (HEK293) and predicts that AKAPs have access to and avidly bind cytoplasmic type II PKAs, and changes in the level of AKAP can alter the intracellular distribution of type IIPKAs.
Journal ArticleDOI
Enhanced secretion of TIMP-1 by human hypertrophic scar keratinocytes could contribute to fibrosis
Franck Simon,Daniele Bergeron,Sébastien Larochelle,Carlos A. Lopez-Vallé,Hervé Genest,Alexis Armour,Véronique J. Moulin +6 more
TL;DR: The findings of this study represent an important advance in understanding the role of keratinocytes as a direct potent modulator for matrix degradation and scar tissue remodeling, possibly through inactivation of MMPs.
Journal ArticleDOI
Purification and characterization of extracellular pectate lyase from Bacillus subtilis.
TL;DR: Bacillus subtilis strain SO113 secretes a pectate lyase which is produced during the exponential death phase of growth, just before sporulation and it was shown that pECTate lyases b and c of this strain are immunologically closely related to the Bacillus subtillis pectates.
Journal ArticleDOI
Histochemistry and biochemistry of tartrate-resistant acid phosphatase (TRAP) and tartrate-resistant acid adenosine triphosphatase (TrATPase) in bone, bone marrow and spleen: implications for osteoclast ontogeny.
TL;DR: Comparison of the biochemically measured TRAP and TrATPase activities showed that bone had the highest specific activity for both enzymes followed by the bone marrow and spleen, however, enzyme activity in the spleen compared to bone was about 4-fold higher for TRAP compared to TratPase.
Journal ArticleDOI
Purification and characterization of human lipoprotein lipase and hepatic triglyceride lipase. Reactivity with monoclonal antibodies to hepatic triglyceride lipase.
TL;DR: Several epitopes of the hepatic triglyceride lipase protein moiety are not present in the lipoprotein lipase molecule, strongly supporting the view that the two enzymes are different proteins.
References
More filters
Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
Journal ArticleDOI
Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
Journal ArticleDOI
Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.