Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
Reads0
Chats0
TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
Citations
More filters
Journal ArticleDOI
Beta-succinyl-coenzyme A synthetase from Trichomonas vaginalis is a soluble hydrogenosomal protein with an amino-terminal sequence that resembles mitochondrial presequences.
TL;DR: Studies directed toward understanding the biogenesis and origin of the hydrogenosome, an unusual organelle found exclusively in certain anaerobic eukaryotes that lack mitochondria, are described.
Journal ArticleDOI
Purification and properties of cyclic AMP-independent glycogen synthase kinase 1 from rabbit skeletal muscle.
TL;DR: A cyclic AMP-independent casein (phosvitin) kinase eluted from a phosphocellulose column with 0.35 M KCl also possesses glycogen synthase kinase activity, and it is suggested that these three kinase activities reside in a single protein.
Journal ArticleDOI
Expression of Brassica juncea 3-hydroxy-3-methylglutaryl CoA synthase is developmentally regulated and stress-responsive.
TL;DR: HMGS assays confirmed that Escherichia coli-expressed recombinant BjHMGS1 shows HMGS activity that is inhibited by F244, a specific inhibitor of HMGS, suggesting that, like HMGR, HMGS is involved in defence.
Journal ArticleDOI
(S)-norlaudanosoline synthase: The first enzyme in the benzylisoquinoline biosynthetic pathway
Martina Rueffer,Martina Rueffer,Hannemarie El-Shagi,Hannemarie El-Shagi,Naotaka Nagakura,Naotaka Nagakura,Meinhart H. Zenk,Meinhart H. Zenk +7 more
TL;DR: In this article, the initial reaction in isoquinoline biosynthesis has long been assumed to be a condensation of two aromatic units, both derived from tyrosine, namely dopamine and 3,4-d~ydroxyphenylacet~dehyde.
Journal ArticleDOI
The rate of ATP synthesis by reconstituted CF0F1 liposomes
Günter Schmidt,Peter Gräber +1 more
TL;DR: The conditions for reconstitution of CF0F1 into asolectin liposomes and for ATP synthesis driven by an artificially generated Δ\gmH+ were optimized and a rate of maximally 200 ATP per CF0f1 per s was obtained with a multi-mixing quenched-flow technique.
References
More filters
Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
Journal ArticleDOI
Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
Journal ArticleDOI
Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.