Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
Citations
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Drosophila cAMP-dependent protein kinase.
TL;DR: Three regions of the Drosophila genome were found which, when present in three copies, significantly alter the specific activity of cAMP-dependent protein kinase.
Journal ArticleDOI
Induction of glucose oxidase, catalase, and lactonase in Aspergillus niger
C.F.B. Witteveen,P.J.I. van der Vondervoort,H.C. van den Broeck,F.A.C. van Engelenburg,L.H. de Graaff,M.H.B.C. Hillebrand,Peter J. Schaap,Jaap Visser +7 more
TL;DR: It was shown that both glucose and oxygen effects reflect regulation at the transcriptional level and it was concluded that the goxC marker which is located on chromosome 2 represents the structural gene of glucose oxidase.
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Carbon and nitrogen metabolism in the seagrass, Zostera marina L.: Environmental control of enzymes involved in carbon allocation and nitrogen assimilation
TL;DR: Overall, the enzymatic responses that were observed indicate a tight coupling between carbon and nitrogen metabolism that is strongly influenced by prevailing environmental conditions, especially temperature, salinity, and environmental nutrient levels.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
Journal ArticleDOI
Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.