Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
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Diacylglycerol kinase from pig brain. Purification and phospholipid dependencies.
TL;DR: The purified diacylglycerol kinase showed a single protein band on polyacrylamide gel electrophoresis in the presence and absence of sodium dodecyl sulfate and the activity of the purified enzyme was markedly enhanced by either deoxycholate or phospholipids.
Journal ArticleDOI
Neuroprotective effect of blackberry (Rubus sp.) polyphenols is potentiated after simulated gastrointestinal digestion
Lucélia Tavares,Inês Figueira,Diana Macedo,Gordon J. McDougall,Maria C. Leitão,Helena L. A. Vieira,Derek Stewart,Paula M. Alves,Ricardo B. Ferreira,Ricardo B. Ferreira,Cláudia N. Santos +10 more
TL;DR: Digested blackberry metabolites protected neuroblastoma cells from H 2 O 2 -induced death at low, non-toxic levels that approach physiologically-relevant serum concentrations, however, the original extracts were not protective even at fivefold higher concentrations.
Journal ArticleDOI
Microtubule-associated polypeptides tau are altered in Alzheimer paired helical filaments.
Inge Grundke-Iqbal,Andrzej W. Vorbrodt,Khalid Iqbal,Yunn-Chyn Tung,G. P. Wang,Henryk M. Wisniewski +5 more
TL;DR: Antisera were raised in rabbits to purified bovine tau and to isolated Alzheimer paired helical filaments (PHF) washed with sodium dodecyl sulfate and Absorption of tau antisera with t Tau and PHF and of PHF antisersa with PHF resulted in complete removal of the tangles-staining antibodies.
Journal ArticleDOI
Synapsin III: Developmental Expression, Subcellular Localization, and Role in Axon Formation
TL;DR: The results indicate that synapsin III is expressed early during development, with levels peaking 7 d after plating and declining thereafter, and has a developmental time course, a subcellular localization, and a developmental function very different from those of synapin I andsynapsin II.
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The inhibitory guanine nucleotide-binding protein (Ni) purified from bovine brain is a high affinity GTPase.
G Milligan,W A Klee +1 more
TL;DR: A pertussis toxin substrate with the expected characteristics of the inhibitory guanine nucleotide-binding protein (Ni) essentially to homogeneity is purified and is able to restore opiate-mediated inhibition of adenylate cyclase to membranes prepared from NG 108-15 cells which had been treated with pertussi toxin.
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.