Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
Citations
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A simple, economical method for measuring bacterial protein synthesis rates in seawater using 3H-leucine
David C. Smith,Farooq Azam +1 more
TL;DR: A simplified method for using 3H-leucine incorporation to measure bacterial protein synthesis rates in seawater is presented in this paper, which uses small volumes (< 2.0 ml) of seawater and centrifugation instead of filtration.
Journal ArticleDOI
Enhanced macrophage degradation of low density lipoprotein previously incubated with cultured endothelial cells: recognition by receptors for acetylated low density lipoproteins.
TL;DR: It is shown that endothelial cells have the potential to metabolically modify the LDL molecule, generating a form that is more rapidly degraded by macrophages and that is recognized by the macrophage receptor for acetylated LDL.
Journal ArticleDOI
Hyperphosphorylation induces self-assembly of τ into tangles of paired helical filaments/straight filaments
TL;DR: It appears that τ self-assembles by association of the microtubule binding domains/repeats and that the abnormal hyperphosphorylation promotes the self-assembly of τ into tangles of PHF and straight filaments by neutralizing the inhibitory basic charges of the flanking regions.
Journal ArticleDOI
Alzheimer's disease hyperphosphorylated tau sequesters normal tau into tangles of filaments and disassembles microtubules
TL;DR: In solution normal tau associates with the AD hyperphosphorylated tau in a nonsaturable fashion, forming large tangles of filaments 3.3 ± 0.7 nm in diameter, which provide insight into how the hyperph phosphorylation of tau might lead to the formation of the neurofibrillary tangles and the degeneration of the affected neurons in AD.
Book ChapterDOI
Protein determination in membrane and lipoprotein samples: manual and automated procedures.
TL;DR: By adding sodium dodecyl sulfate to the alkali reagent, samples can be assayed directly without prior solubilization or delipidation and quantitation of protein in the presence of sucrose and EDTA is facilitated.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
Journal ArticleDOI
Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
Journal ArticleDOI
Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.