Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
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Bacteriochlorophyll-protein complexes from the light-harvesting antenna of photosynthetic bacteria.
Kenneth Sauer,Lynn A. Austin +1 more
TL;DR: Detergent-solubilized bacteriochlorophyll--protein complexes corresponding to the light-harvesting antenna are isolated from Rhodopseudomonas sphaeroides, strain 2.4.1 and R-2 (carotenoidless) mutant, and from Rhodospirillum rubrum, wild-type.
Journal ArticleDOI
Alzheimer paired helical filaments Restoration of the biological activity by dephosphorylation
TL;DR: It is suggested that in Alzheimer disease tau in PHF is functionally inactive because of abnormal phosphorylation and that the abnormally phosphorylated site(s) inPHF that inactivates PHF‐tau is accessible to enzymatic dephosphorylation in vitro.
Journal ArticleDOI
Binding of lipoprotein lipase to endothelial cells in culture.
TL;DR: Equilibrium-binding data of highly purified avian lipoprotein lipase to cultured bovine endothelial cells demonstrate the presence of a class of high affinity sites and the demonstration that the preparation utilized contained no detectable protease activity is further evidence that lipop protein lipase is bound to endothelial cell heparan sulfate or heparAn sulfate-like molecules.
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Inhibitory effects of quinolone antibacterial agents on gamma-aminobutyric acid binding to receptor sites in rat brain membranes.
TL;DR: The findings suggest that the inhibition of GABA binding to receptors (including uptake sites) in the brain may be involved in the induction of epileptogenic neurotoxicities by quinolones.
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Seasonally fluctuating bark proteins are a potential form of nitrogen storage in three temperate hardwoods.
TL;DR: The high levels of specific proteins in inner bark tissues and the presence of protein bodies within the parenchyma cells indicate that the living cells of the bark act as a nitrogen reserve in overwintering temperate hardwoods.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
Journal ArticleDOI
Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.