Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
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The soybean 94-kilodalton vegetative storage protein is a lipoxygenase that is localized in paraveinal mesophyll cell vacuoles.
TL;DR: Observations suggest that plant lipoxygenases may be bifunctional proteins able to function enzymatically in the hydroperoxidation of lipids and also to serve a role in the temporary storage of nitrogen during vegetative growth.
Journal ArticleDOI
Regulation of Ca2+-pumping ATPase of heart sarcolemma by a phosphorylation-dephosphorylation Process.
P Caroni,E Carafoli +1 more
TL;DR: The Ca2+ dependence curve of the Ca2-ATPase uptake in phospho- and dephosphorylated vesicles suggests that the phosphorylation might affect the efficiency of the enzyme (turnover rate) rather than its affinity for Ca2+.
Journal ArticleDOI
Rat calcium-binding proteins: distribution, development, and vitamin D dependence.
TL;DR: It is demonstrated that the tissue distribution of the two CaBPs is different in the rat, and high levels of the 28 H protein were found in the cerebellum and kidney, whereas the smaller CaBP was concentrated in the duodenum, jejunum, and cecum.
Journal ArticleDOI
Specific binding of a fungal glucan phytoalexin elicitor to membrane fractions from soybean Glycine max
Walter E. Schmidt,Jürgen Ebel +1 more
TL;DR: Competition studies with the [(3)H]glucan elicitor and a number of polysaccharides demonstrate that only poly Saccharides of a branched beta-glucAn type effectively displace the radiolabeled ligand from membrane binding.
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DNA binding and photocleavage in vitro by new dirhodium(II) dppz complexes: correlation to cytotoxicity and photocytotoxicity.
Alfredo M. Angeles-Boza,Patricia M. Bradley,Patty K.-L. Fu,Sara E. Wicke,John Bacsa,Kim R. Dunbar,Claudia Turro +6 more
TL;DR: The results suggest that intercalation or strong DNA binding may not be a desirable property of a potential PDT agent.
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.