Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
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The chloroplast ATP synthase in Chlamydomonas reinhardtii: I. Characterization of its nine constitutive subunits
TL;DR: A 70-kDa peripheral membrane protein in the thylakoid membranes of C. reinhardtii is identified, which is immunologically related to the beta subunit of CF1, and its conceivable ATPase function with respect to the Ca2+-dependent ATPase activity is discussed.
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Interaction of rhodanese with mitochondrial NADH dehydrogenase
Silvia Pagani,Y.M. Galante +1 more
TL;DR: Rhodanese-mediated sulfide transfer was directly demonstrated when the reactivation of NADH dehydrogenase was performed in the presence of radioactive thiosulfate and the 35S-loaded flavoprotein was re-isolated by gel filtration chromatography, indicating that the [35S]sulfide was inserted in NADH dehydration and appeared to constitute the structural basis for the increase in enzymic activity.
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A Light-dependent Protein Kinase Activity of Chloroplasts.
TL;DR: A protein kinase activity from spinach chloroplasts, tightly associated with the thylakoid membranes, has been solubilized and partially characterized and remains active in the dark after short illumination periods, suggesting that a product of electron transport may be involved in light activation.
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Purification of insulin-like growth factor I receptor from human placental membranes.
TL;DR: Insulin-like growth factor (IGF) I receptor was purified from Triton X-100-solubilized human placental membranes by wheat germ agglutinin-Sepharose chromatography followed by immunoaffinity chromatography using alpha IR-3, a monoclonal antibody directed against the IGF-I receptor.
Journal ArticleDOI
In vitro inhibition of midazolam and quinidine metabolism by flavonoids.
TL;DR: The data suggest that the flavonoids found in grapefruit juice may influence the kinetics of midazolam and quinidine in man.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.