Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
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Purification and biochemical characterization of a putative [6Fe-6S] prismane-cluster-containing protein from Desulfovibrio vulgaris (Hildenborough).
TL;DR: This work believes that it has encountered the first example of a prismane-cluster-containing protein, isolated from the sulfate-reducing bacterium Desulfovibrio vulgaris, and cross-reactivity of antibodies against this protein indicated the occurrence of a similar protein in D. vulgaris (Monticello).
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Ectoenzymes of the kidney microvillar membrane. Affinity purification, characterization and localization of the phospholipase C-solubilized form of renal dipeptidase
TL;DR: Results establish renal dipeptidase as a glycosyl-phosphatidylinositol-anchored ectoenzyme of the microvillar membrane.
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The Dictyostelium discoideum 30,000-dalton protein is an actin filament-bundling protein that is selectively present in filopodia.
TL;DR: The interaction with actin and intracellular localization of the 30,000- D actin-binding protein from the cellular slime mold Dictyostelium discoideum have been investigated to analyze the potential contributions of this protein to cell structure and movement and suggest that the 30-000-D protein contributes to organization of bundles of actin filaments in filopodia.
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Structural features of a pectic arabinogalactan with immunological activity from the leaves of Diospyros kaki.
TL;DR: It was proposed that the acid-labile arabinofuranosyl residues in the side chains would not be needed for the expression of the enhancement of the immunological activity, and that the presence of GalAp in the backbone has an important, but not crucial effect on theexpression of the activity.
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Ribonucleic acid and other polyanions facilitate chromatin assembly in vitro
TL;DR: The ability of Drosophila embryo extracts to mediate chromatin assembly in vitro requires an anionic fraction which the authors demonstrate to be RNA, and exogenous natural and homopolymer RNAs, if of sufficient length, can also mediate ChromatinAssembly in vitro.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.