Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
Citations
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Light-controlled expression of a gene encoding l-galactono-γ-lactone dehydrogenase which affects ascorbate pool size in Arabidopsis thaliana
Masanori Tamaoki,Fumiko Mukai,Naoko Asai,Nobuyoshi Nakajima,Akihiro Kubo,Mitsuko Aono,Hikaru Saji +6 more
TL;DR: The results suggest that the diurnal change in expression of AtGLDH, which may play an important role in the regulation of AsA pool size, is regulated not by a circadian rhythm but by light.
Journal ArticleDOI
Sex-specific regulation of yolk protein gene expression in Drosophila.
TL;DR: Temperature shift experiments with a temperature-sensitive allele of the sex determination regulatory gene transformer-2 showed that tra-2+ function is required in the adult for both the sex-specific initiation and maintenance of YP synthesis, providing a paradigm for the way this regulatory hierarchy controls the terminal differentiation functions for sexually dimorphic development.
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EPR studies of iron-sulfur clusters in isolated subunits and subfractions of NADH-ubiquinone oxidoreductase.
TL;DR: A tentative scheme for the spatial organization of these iron-sulfur clusters in the enzyme and in the membrane is proposed by combining information on subunit topography and spin-spin interactions between redox centers in the native enzyme.
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Quantification of protein thiols and dithiols in the picomolar range using sodium borohydride and 4,4'-dithiodipyridine
TL;DR: A quick and highly sensitive assay for protein thiol and dithiol quantification using the reducing agent sodium borohydride and the thiol reagent 4,4'-dithiodipyridine (4-DPS).
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Identification of the major cytochrome P-450 form transplacentally induced in neonatal rabbits by 3,3,7,8-tetrachlorodibenzo-p-dioxin.
TL;DR: It is demonstrated that microsomal metabolism in TCDD-treated newborn rabbits reflects the catalytic properties of the predominant cytochrome P450 species, which is form 6.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.