Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
Citations
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Journal ArticleDOI
Rapid stimulation by insulin of a serine/threonine kinase in 3T3-L1 adipocytes that phosphorylates microtubule-associated protein 2 in vitro
L B Ray,T W Sturgill +1 more
TL;DR: Results show that a soluble serine/threonine kinase is rapidly activated by insulin, possibly by phosphorylation of either the kinase itself or an interacting modulator.
Journal ArticleDOI
Drug- and estrogen-induced cholestasis through inhibition of the hepatocellular bile salt export pump (Bsep) of rat liver.
TL;DR: Bsep is identified as an important target for induction of drug- and estrogen-induced cholestasis in mammalian liver through ATP-dependent taurocholate transport in cLPM vesicles.
Journal ArticleDOI
The Plant Cell Wall–Decomposing Machinery Underlies the Functional Diversity of Forest Fungi
Daniel C. Eastwood,Dimitrios Floudas,Manfred Binder,Andrzej Majcherczyk,Patrick Schneider,Andrea Aerts,Fred O. Asiegbu,Scott E. Baker,Kerrie Barry,Mika Bendiksby,Melanie Blumentritt,Pedro M. Coutinho,Dan Cullen,Ronald P. de Vries,Allen C. Gathman,Barry Goodell,Barry Goodell,Bernard Henrissat,Katarina Ihrmark,Håvard Kauserud,Annegret Kohler,Kurt LaButti,Alla Lapidus,José Luis Lavín,Yong-Hwan Lee,Erika Lindquist,Walt W. Lilly,Susan Lucas,Emmanuelle Morin,Claude Murat,José A. Oguiza,Jongsun Park,Antonio G. Pisabarro,Robert Riley,Anna Rosling,Asaf Salamov,Olaf Schmidt,Jeremy Schmutz,Inger Skrede,Jan Stenlid,Ad Wiebenga,Xinfeng Xie,Ursula Kües,David S. Hibbett,Dirk Hoffmeister,Nils Högberg,Francis Martin,Igor V. Grigoriev,S.C. Watkinson +48 more
TL;DR: Fungal nutritional mode diversification suggests that the boreal forest biome originated via genetic coevolution of above- and below-ground biota through convergent evolution and divergence among fungal decomposers.
Book ChapterDOI
Measurement of growth and viability of cells in culture.
TL;DR: This chapter focuses on cell culture measurements that can be divided into four major categories: visual methods, which employ the use of light microscopy and devices commonly used in hematology; chemical methods,which employ commonly used analytical biochemical procedures adapted to tissue culture; electronic systems using flow-through cells or apertures for measurement of incorporated dyes or cell numbers; and an array of miscellaneous procedures.
Journal ArticleDOI
Purification and characterization of a unique isozyme of cytochrome P-450 from liver microsomes of ethanol-treated rabbits.
TL;DR: Peptide mapping by sodium dodecyl sulfate-polyacrylamide gel electrophoresis following treatment with papain, chymotrypsin, or Staphylococcus aureus V8 protease and by high performance liquid chromatography following trypsinolysis indicates that form 3a is a unique gene product.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
Journal ArticleDOI
Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
Journal ArticleDOI
Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.