Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
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Phenylalanine hydroxylase from Chromobacterium violaceum is a copper-containing monooxygenase. Kinetics of the reductive activation of the enzyme.
TL;DR: The 4a-hydrate of 6-methyltetrahydropterin (4a-carbinolamine) is observed during turnover of the enzyme, suggesting that the mechanism of oxygen activation is similar for both enzymes.
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Light and Cytokinin Play a Co-operative Role in MGDG Synthesis in Greening Cucumber Cotyledons
Yoshiki Yamaryo,Daisuke Kanai,Koichiro Awai,Mie Shimojima,Tatsuru Masuda,Hiroshi Shimada,Ken-ichiro Takamiya,Hiroyuki Ohta +7 more
TL;DR: The results suggest that galactolipid biosynthesis is regulated by the cooperation of light and a cytokinin, and that a red light dependent factor is necessary for the maximal galactosyldiacylglycerol accumulation in addition to increase in MGD transcript and activity.
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The glycosylation of the aspartic proteinases from barley (Hordeum vulgare L.) and cardoon (Cynara cardunculus L.).
Júlia Costa,David A. Ashford,Manfred Nimtz,Isabel Bento,Carlos Frazão,Cristina L. Esteves,Carlos Faro,Jukka Kervinen,Euclides Pires,Paula Veríssimo,Alexander Wlodawer,Maria Arménia Carrondo +11 more
TL;DR: The glycosylation sites are occupied for the barley (Hordeum vulgare L.) aspartic proteinase and the cardoon, cardosin A (Asn70 and Asn363) and their structures were determined by exoglycosidase sequencing combined with matrix-assisted laser desorption/ionization time of flight mass spectrometry.
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Agrin Differentially Regulates the Rates of Axonal and Dendritic Elongation in Cultured Hippocampal Neurons
TL;DR: An important role for agrin is suggested as a trigger of the transcription of neuro-specific genes involved in neurite elongation and branching in central neurons.
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Inhibiting Δ9-Desaturase Activity Impairs Triacylglycerol Secretion in Cultured Chicken Hepatocytes
TL;DR: Results show that TG secretion is highly dependent on the delta9-desaturase activity and suggest that oversecretion of VLDL-TG in chickens and subsequent fattening could originate in a high hepatic delta 9-desaturation of saturated fatty acids.
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.