Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
Citations
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The Small Regulatory RNA SyR1/PsrR1 Controls Photosynthetic Functions in Cyanobacteria
Jens Georg,Dennis Dienst,Nils Schürgers,Thomas Wallner,Dominik Kopp,Damir Stazic,Ekaterina Kuchmina,Stephan Klähn,Heiko Lokstein,Wolfgang R. Hess,Annegret Wilde +10 more
TL;DR: Computational and experimental data consistently establish the small RNA PsrR1 as a regulatory factor controlling photosynthetic functions.
Journal ArticleDOI
Identification of the essential tyrosine residue in the beta subunit of bovine heart mitochondrial F1-ATPase that is modified by 7-chloro-4-nitro[14C]benzofurazan.
TL;DR: Inactivation of the bovine heart mitochondrial F1-ATPase, taken as alpha 3 beta 3 gamma delta epsilon with a molecular weight of 375,000, with a 4-fold molar excess of 7-chloro-4-nitro[14C]benzofurazan at pH 7.5, led to the incorporation of 1.42 g atoms of 14C/mol.
Journal ArticleDOI
Oxidation of trichloroethylene and dimethyl sulfide by a marine Methylomicrobium strain containing soluble methane monooxygenase.
Hiroyuki Fuse,Misaki Ohta,Osamu Takimura,Katsuji Murakami,Hiroyuki Inoue,Yukiho Yamaoka,Jose M. Oclarit,Toshio Omori +7 more
TL;DR: Strain NI degraded trichloroehylene (TCE) more efficiently than other methanotrophic isolates containing no sMMO only under copper limiting conditions and phylogenetic analysis based on 16S rDNA genes indicated that strain NI was a type I meethanotroph belonging to the genus Methylomicrobium, and closely related to Methylombium pelagicum.
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Purification and characterization of the berberine bridge enzyme from berberis beaniana cell cultures
TL;DR: The berberine bridge-forming enzyme (BBE) has been found in 66 samples taken from differentiated plants and from cell suspension cultures and was shown to be homogeneous by gel electrophoresis.
Journal ArticleDOI
Purification and characterization of a ribosome dissociation factor (eukaryotic initiation factor 6) from wheat germ.
TL;DR: A wheat germ ribosome dissociation factor, eIF-6, has been purified almost to homogeneity from the 25 to 40% ammonium sulfate fraction of the postribosomal supernatant and its chromatographic properties permit its separation from the known wheat germ initiation factors.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.