Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
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Determination and characterization of cross‐reacting allergens in latex, avocado, banana, and kiwi fruit
TL;DR: Almost all IgE‐reactive bands in nitrocellulose‐blotted latex, avocado, and banana extracts and two components of 43 and 67 kDa in kiwi fruit shared common IgE epitopes, and cross‐reactions between these allergen extracts were determined by EAST inhibition.
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Characterization of Xylanolytic Enzymes in Clostridium cellulovorans: Expression of Xylanase Activity Dependent on Growth Substrates
TL;DR: Results strongly indicated that C. cellulovorans is able to regulate the expression of xylanase activity and to vary the cellulosome composition depending on the growth substrate.
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Purification and characterization of highly active and stable polyphosphatase from Saccharomyces cerevisiae cell envelope.
TL;DR: Saccharomyces cerevisiae cell envelope polyphosphatase was isolated in highly active and stable form by extraction from cells with zwittergent TM‐314 and after 30 days retained 87% of its activity at −20°C.
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Localization of catalytic and regulatory subunits of cyclic AMP-dependent protein kinases in mitochondria from various rat tissues
TL;DR: Immunoblot analysis of subfractions from liver mitochondria supported the localization in situ of cyclic AMP-dependent protein kinases in the inner membrane/matrix space and suggested that the type I enzyme is anchored by its regulatory subunit to the outer membrane.
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Detailed characterization of cysteine-less P-glycoprotein reveals subtle pharmacological differences in function from wild-type protein.
Andrew M Taylor,Janet Storm,Loredana Soceneantu,Kenneth J. Linton,Mark P. Gabriel,Catherine Martin,James Woodhouse,Emma J. Blott,Christopher F. Higgins,Richard Callaghan +9 more
TL;DR: The serine to cysteine mutations in P‐gp may suggest that vinblastine and nicardipine transduce their effects on ATP hydrolysis through distinct conformational pathways, and the wt and cys‐less P‐GP isoforms display similarity in their fundamental kinetic properties thereby validating the use of cys-less P-gp as a template for future cysteINE‐directed structure/function analysis.
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.