Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
Citations
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Inhibition of ribulose-1,5-bisphosphate carboxylase/oxygenase by ribulose-1,5-bisphosphate epimerization and degradation products
TL;DR: All ribulose-P2 generated in situ may be stored as this complex to prevent decomposition, as the KD of the enzyme/substrate complex is ≤1 μM, and one reason for a high level of ribULose- P2 carboxylase in chloroplasts where the molarity of active sites exceeds that of ribuloses P2.
Journal ArticleDOI
Hydroxylated polychlorinated biphenyls are substrates and inhibitors of human hydroxysteroid sulfotransferase SULT2A1.
TL;DR: The interactions of OHPCBs with human SULT2A1 represent both a potential route of metabolism and a possible source of interference with sulfation reactions catalyzed by this enzyme.
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A comparison of molecular properties of hepatic triglyceride lipase and lipoprotein lipase from human post-heparin plasma.
TL;DR: The differences previously described regarding optima of pH and ionic strength and the requirement for apolipoprotein CII (only for lipoprotein lipase) were maintained in the highly purified state.
Journal ArticleDOI
The cytosolic factor required for import of precursors of mitochondrial proteins into mitochondria.
TL;DR: From results, the mechanism of binding of the extrapeptide to the receptor of the mitochondrial outer membrane is suggested to be as follows: the peptide (the precursor of mitochondrial protein) and the cytosolic factor form a complex, and then the complex is recognized by and bound to the receptors.
Journal ArticleDOI
Structure characterization of a novel neutral polysaccharide isolated from Ganoderma lucidum fruiting bodies
Deng Pan,Linqiang Wang,Congheng Chen,Bao-Song Teng,Chen-Dong Wang,Zhi-Xue Xu,Bing-Wen Hu,Ping Zhou +7 more
TL;DR: The results of monosaccharide composition and GPC analysis indicated that FYGL-1 consisted of galactose, rhamnose and glucose in mole ratio of 1.00:1.22 with a molecular weight of 78kDa.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.